Skripsi

Bioactivity Testing of Ethyl Acetate and n-Hexane Extracts of Neem Leaves as Antifungal Candidates against Malassezia globosa = Uji Bioaktivitas Ekstrak Etil Asetat dan Heksan Daun Mimba Sebagai Kandidat Antijamur Malassezia globosa.

Introduction Due to the adverse effects related to the long-term use of anti-malassezia drugs such as ketoconazole, an alternative medication should be determined. Method The purpose of this study is to determine the growth inhibition effect of neem (Azadirachta indica) leaves n-hexane and ethyl acetate extract at 200, 250, 300, 350, 400 and 450 ppm against Malassezia globosa through broth microdilution after incubation for 72 hours, as well as each hour after (73-78). Results Data is obtained through ELISA, which is analyzed through absorbance trend, GraphPad, and SPSS by One-Way ANOVA, Tukey’s post hoc test, Friedman test, and DunnBonferroni post hoc test. Conclusion Results found that both n-hexane (350-400 ppm) and ethyl acetate (200, 350, and 400 ppm) were able to inhibit Malassezia globosa growth, however there is no significant difference found between each concentration.
Keywords: Malassezia globosa, Azadirachta indica, broth microdilution, ketoconazole, absorbance value


Latar Belakang Dikarenakan efek samping dari penggunaan jangka panjang obat anti-malassezia seperti ketokonazol, pengobatan alternatif harus ditemukan. Metode Tujuan dari studi ini adalah untuk menentukan efek penghambatan pertumbuhan dari daun mimba (Azadirachta indica) dalam bentuk ekstrak heksan dan etil asetat dalam dosis 200, 250, 300, 350, 400 dan 450 ppm terhadap Malassezia globosa pada mikrodilusi kaldu setelah inkubasi selama 72 jam, serta setiap satu jam setelahnya (73-78). Hasil Data didapatkan melalui ELISA, dengan analisis tren absorbansi, GraphPad, dan SPSS menggunakan One-Way ANOVA, uji post hoc Tukey, uji Friedman, dan uji post hoc Dunn-Bonferroni. Kesimpulan Hasil menunjukkan bahwa ekstrak heksan (350-400 ppm) dan etil asetat (200, 350, dan 400 ppm) dapat menghambat pertumbuhan Malassezia globosa, walaupun tidak ada perbedaan yang signifikan antara konsentrasi-konsentrasi yang ada.
Kata Kunci: Malassezia globosa, Azadirachta indica, mikrodilusi kaldu, ketokonazol, nilai absorbansi