Skripsi

Analisis Kadar Katalase pada Ginjal Tikus Nefropati Obstruktif yang Diberikan 6-Gingerol Latar Belak = Analysis of Catalase Levels in the Kidneys of Obstructive Nephropathy Rats Treated with 6-Gingerol.

Latar Belakang Penyakit ginjal kronis (PGK) merupakan salah satu penyebab utama kematian di dunia. Salah satu faktor yang dapat memicu terjadinya PGK adalah obstruksi saluran kemih, yang menyebabkan peningkatan stres oksidatif. Kondisi ini berdampak pada terhambatnya aktivitas enzim antioksidan, seperti katalase. Katalase dapat digunakan sebagai parameter stres oksidatif karena merupakan salah satu antioksidan utama di dalam sel. Senyawa 6-gingerol, komponen aktif utama dalam jahe (Zingiber officinale), diketahui mampu meningkatkan aktivitas enzim antioksidan sehingga berpotensi digunakan sebagai terapi untuk mengatasi stres oksidatif. Namun, hingga saat ini belum ada penelitian yang mereplikasi efek tersebut pada ginjal dengan kondisi nefropati obstruktif. Metode Tikus Sprague-Dawley jantan dibagi secara acak ke dalam tiga kelompok perlakuan: Kontrol (diet konvensional), UUO (ligasi ureter kiri + diet konvensional), dan Intervensi (ligasi ureter kiri + 6-gingerol dosis 200 mg/kg). Setelah 2 minggu perlakuan sesuai kelompoknya, ginjal kiri diambil, lalu dibuat homogenat untuk menentukan kadar protein dan katalasenya. Efek nefropati obstruktif dan pemberian 6-gingerol dikuantifikasi dengan kadar katalase, yang diukur dengan metode enzyme-linked immunosorbent assay (ELISA). Hasil Tidak terdapat perbedaan bermakna kadar katalase antarkelompok (p = 0,138). Kesimpulan Pemberian 6-gingerol tidak meningkatkan kadar katalase pada ginjal tikus nefropati obstruktif.
Kata Kunci: 6-gingerol, katalase, nefropati obstruktif, unilateral ureteral obstruction


Introduction Chronic kidney disease (CKD) is one of the leading causes of death worldwide. One of the factors that can trigger CKD is urinary tract obstruction, which induces an increase in oxidative stress. This condition results in the inhibition of antioxidant enzyme activity, such as catalase. Catalase can be used as a parameter of oxidative stress because it is one of the main intracellular antioxidants. 6-Gingerol, the main active component of ginger (Zingiber officinale), has been reported to enhance antioxidant enzyme activity and therefore has potential as a therapeutic agent against oxidative stress. However, to date, no studies have replicated this effect in kidneys with obstructive nephropathy. Method Male Sprague-Dawley rats were randomly divided into three treatment groups: Control (standard diet), UUO (left ureteral ligation + standard diet), and Intervention (left ureteral ligation + 6-gingerol at a dose of 200 mg/kg). After two weeks of treatment, the left kidney was collected and homogenized to determine protein concentration and catalase activity. The effects of obstructive nephropathy and 6-gingerol administration were quantified based on catalase levels, which were measured using the enzyme-linked immunosorbent assay (ELISA) method. Results There was no significant difference in catalase levels between groups (p = 0,138). Conclusion Administration of 6-gingerol did not increase catalase levels in the kidneys of rats with obstructive nephropathy.
Keywords: 6-gingerol, catalase, obstructive nephropathy, unilateral ureteral obstruction