Skripsi

Analisis Kadar Glutathione Peroxidase (GPx) pada Ginjal Tikus Nefropati Obstruktif yang Diberikan 6-Gingerol = Analysis of Glutathione Peroxidase (GPx) Levels in the Kidneys of Obstructive Nephropathy Rats Treated with 6-gingerol.

Latar Belakang Nefropati obstruktif adalah penyakit ginjal akibat obstruksi saluran kemih yang berlangsung kronis. Proses patologis yang mendasari perkembangan penyakit nefropati obstruktifadalah stres oksidatif, yang dapat ditandai dengan penurunan enzim glutathione peroxidase (GPx). Salah satu senyawa aktif jahe, yaitu 6-gingerol terbukti memiliki sifat antioksidan dan berpotensi sebagai obat untuk mengatasi nefropati obstruktif. Penelitian ini bertujuan untuk menganalisis pengaruh pemberian 6-gingerol pada kadar GPx ginjal tikus. Metode Tikus unilateral ureteral obstruction (UUO) merupakan model eksperimental yang dapat menggambarkan nefropati obstruktif. Tikus Sprague-Dawley jantan dibagi menjadi 3 kelompok, yaitu kelompok normal, kelompok UUO, dan kelompok UUO yang diberi 6- gingerol dosis 200 mg/kgBB secara oral selama 2 minggu. Pengukuran GPx dari homogenat ginjal tikus dilakukan dengan kit Enzyme-linked Immunosorbent Assay (ELISA). Hasil Hasil menunjukkan bahwa kadar GPx tikus normal adalah 0,238 ± 0,082 ng/mg, tikus UUO 0,145 ± 0,015 ng/mg, dan tikus UUO + 6-gingerol 0,168 ± 0,032 ng/mg. Tikus UUO mengalami penurunan kadar GPx yang signifikan (p = 0,018). Namun, pemberian 6-gingerol tidak dapat memberikan peningkatan kadar GPx yang signifikan. Kesimpulan Pemberian 6-gingerol 200 mg/kgBB selama 2 minggu tidak dapat meningkatkan kadar GPx ginjal tikus nefropati obstruktif secara signifikan.
Kata Kunci: 6-gingerol, glutathione peroxidase, UUO, nefropati obstruktif


Introduction Obstructive nephropathy is a kidney disease caused by chronic urinary tract obstruction. The pathological process underlying the development of obstructive nephropathy is oxidative stress, which can be characterized by a decrease in the enzyme glutathione peroxidase (GPx). One of ginger's active compounds, 6-gingerol, has been shown to have antioxidant properties and has potential as a drug to treat obstructive nephropathy. This study aims to analyze the effect of 6-gingerol administration on renal GPx levels in rats. Method Unilateral ureteral obstruction (UUO) rats are an experimental model that can illustrate obstructive nephropathy. Male Sprague-Dawley rats were divided into three groups: a normal group, a UUO group, and a UUO group treated with 6-gingerol at a dose of 200 mg/kgBW orally for 2 weeks. GPx measurements from rat kidney homogenates were performed using an Enzyme-linked Immunosorbent Assay (ELISA) kit. Results The results showed that GPx levels in normal rats were 0.238 ± 0.082 ng/mg, UUO rats 0.145 ± 0.015 ng/mg, and UUO + 6-gingerol rats 0.168 ± 0.032 ng/mg. UUO rats experienced a significant decrease in GPx levels (p = 0.018). However, administration of 6-gingerol could not provide a significant increase in GPx levels. Conclusion Administration of 6-gingerol 200 mg/kgBB for 2 weeks could not significantly increase the renal GPx levels of nephropathy obstructive rat.
Keywords: 6-gingerol, glutathione peroxidase, UUO, obstructive nephropathy