Skripsi

Analysis Of Sod Levels In The Kidneys Of Obstructive Nephropathy Rats Treated With 6- Gingerol = Analisis Kadar Sod Pada Ginjal Tikus Nefropati Obstruktif Yang Diberikan 6-Gingerol.

Introduction Obstructive nephropathy caused by unilateral ureteral obstruction (UUO) is characterized by oxidative stress and decreased antioxidant defenses, particularly superoxide dismutase (SOD). 6-gingerol, the main bioactive compound in ginger, has been shown to possess antioxidant and renoprotective properties. Method An experimental in vivo study was conducted on male Sprague-Dawley rats, divided into three groups: normal (n=5), UUO (n=6), and UUO + 6-gingerol 200 mg/kgBW/day (n=6) for two weeks. SOD activity in kidney tissue homogenates was measured using ELISA and normalized to protein concentration. Data were analyzed using Shapiro-Wilk, ANOVA, and post hoc tests. Results The mean renal SOD levels were 7.17 ± 1.75 U/mg (normal), 10.94 ± 1.23 U/mg (UUO), and 9.33 ± 1.18 U/mg (UUO + 6-gingerol). ANOVA analysis showed a significant overall difference among groups (p < 0.05). Pairwise comparison revealed a significant increase in SOD activity in the UUO group compared to the normal group (p = 0.001). Conclusion Administration of 6-gingerol (200 mg/kgBW) for two weeks did not significantly increase SOD levels compared to UUO alone. The elevated SOD levels in UUO rats may represent a compensatory response to oxidative stress.
Keywords: obstructive nephropathy, unilateral ureteral obstruction, oxidative stress, superoxide dismutase, 6-gingerol


Latar Belakang Nefropati obstruktif akibat unilateral ureteral obstruction (UUO) ditandai dengan stres oksidatif dan penurunan sistem pertahanan antioksidan, khususnya superoxide dismutase (SOD). 6-gingerol, senyawa bioaktif utama dalam jahe, diketahui memiliki efek antioksidan dan renoprotektif. Penelitian ini bertujuan untuk menganalisis pengaruh pemberian 6-gingerol terhadap kadar SOD ginjal pada tikus nefropati obstruktif model UUO. Metode Penelitian eksperimental in vivo dilakukan pada tikus Sprague-Dawley jantan yang dibagi menjadi tiga kelompok: normal (n=5), UUO (n=6), dan UUO + 6-gingerol 200 mg/kgBB/hari (n=6) selama dua minggu. Aktivitas SOD pada homogenat jaringan ginjal diukur dengan metode ELISA dan dinormalisasi terhadap kadar protein. Data dianalisis menggunakan uji Shapiro-Wilk, ANOVA, dan post hoc. Hasil Rata-rata kadar SOD ginjal adalah 7,17 ± 1,75 U/mg (normal), 10,94 ± 1,23 U/mg (UUO), dan 9,33 ± 1,18 U/mg (UUO + 6-gingerol). Analisis ANOVA menunjukkan perbedaan yang signifikan antar kelompok (p < 0,05). Uji perbandingan berpasangan menunjukkan peningkatan aktivitas SOD yang signifikan pada kelompok UUO dibandingkan dengan kelompok normal (p = 0,001). Kesimpulan Pemberian 6-gingerol (200 mg/kgBB) selama dua minggu tidak secara signifikan meningkatkan kadar SOD dibandingkan UUO saja. Peningkatan SOD pada tikus UUO kemungkinan merupakan respons kompensasi terhadap stres oksidatif.
Kata Kunci: nefropati obstruktif, unilateral ureteral obstruction, stres oksidatif, superoxide dismutase, 6-gingerol