Tesis

Perbandingan Kadar IL-17 Skalp Perempuan Berhijab dengan Dermatitis Seboroik dan Tanpa Dermatitis Seboroik serta Korelasi terhadap Derajat Keparahan = Comparison of Scalp IL-17 Levels in Hijab-Wearing Women with and without Seborrheic Dermatitis and Its Correlation with Disease Severity.

Latar Belakang: Dermatitis seboroik (DS) merupakan kondisi inflamasi kronik kulit berkaitan dengan kolonisasi Malassezia. Pada perempuan berhijab, Malassezia restricta ditemukan meningkat sehingga dikaitkan risiko DS. Seiring peningkatan pengguna hijab di Indonesia, isu ini menjadi relevan. Interleukin (IL)-17 merupakan sitokin proinflamasi yang memiliki peran dalam pertahanan terhadap jamur, termasuk Malassezia. Penelitian peran IL- 17 pada DS, terutama pada populasi khusus berhijab, saat ini belum ada. Tujuan: Mengetahui perbedaan rerata kadar ekspresi IL-17 pada lesi skalp perempuan berhijab dengan dermatitis seboroik dibandingkan skalp normal dan hubungannya dengan derajat keparahan DS. Metode: Penelitian potong lintang ini melibatkan 66 subjek penelitian (SP) perempuan berhijab berusia 18–59 tahun, terdiri dari 34 SP kelompok DS dan 32 SP kelompok tanpa DS setelah SP melewati masa pengkondisian selama 14 hari. IL-17 dinilai dengan metode imunohistokimia pada jaringan skalp yang diambil melalui metode tape stripping. Hasil: Kelompok DS memiliki median kadar IL-17 yang lebih rendah [0,064 (0,006–0,354) pg/µg protein] dibandingkan tanpa DS [0,105 (0,030–0,531) pg/µg protein] yang signifikan secara statistik (p = 0,002). Hal ini didukung dengan korelasi negatif kuat kadar IL-17 terhadap skor derajat keparahan DS (r = -0,728, p = 0,000). Kesimpulan: Kadar IL-17 pada perempuan berhijab dengan DS lebih rendah dibandingkan dengan tanpa DS serta berkorelasi negatif kuat terhadap derajat keparahannya.
Kata kunci: Dermatitis seboroik, IL-17, perempuan berhijab, tape stripping


Background: Seborrheic dermatitis (SD) is a chronic inflammatory skin condition associated with Malassezia colonization. In hijab wearing women, Malassezia restricta has been found to be increased thus linked to a higher risk of SD. With the growing number of hijab users in Indonesia, this issue has gained relevance. Interleukin-17 (IL)-17 is a proinflammatory cytokine that plays a role in antifungal immunity, including against Malassezia. However, studies on the role of IL-17 in SD, particularly in the specific population of hijab wearing women, are currently lacking. Objective: To evaluate the difference in mean IL-17 expression levels in scalp lesions of hijab wearing women with SD compared to normal scalp, and its correlation with disease severity. Methods: This cross-sectional study involved 66 hijab wearing female participants aged 18– 59 years, including 34 participants in the SD group and 32 in the non-SD group. IL-17 expression was assessed using immunohistochemistry on scalp skin samples obtained through tape stripping. Results: The SD group had lower IL-17 levels [0.064 (0.006–0.354) pg/µg protein] compared to the non-SD group [0.105 (0.030–0.531) pg/µg protein] which significant statistically (p = 0.002). This was further supported by a strong negative correlation between IL-17 levels with SD severity scores (r = -0.728, p = 0.000). Conclusion: IL-17 levels in hijab-wearing women with SD were lower than those without SD and showed a strong negative correlation with disease severity.
Key words: Hijab wearing women, IL-17, seborrheic dermatitis, tape stripping