Tesis

Korelasi 25(OH)D Serum dan Kadar Timbal Dalam Darah Terhadap Delta-Aminolevulinic Acid Dehidratase Pekerja Yang Terpajan Timbal = Correlation Between Serum 25(OH)D and Blood Lead Levels to Delta- Aminolevulinic Acid Dehidratase in Lead Exposed Workers.

Latar Belakang Timbal mengganggu jalur sintesis heme dengan memodifikasi aktivitas DeltaAminolevulinic Acid Dehydratase (ALAD), menyebabkan stres oksidatif. Dalam mekanisme ini, vitamin D yang berperan sebagai antioksidan, mungkin memiliki peran dalam mengatur perubahan aktivitas ALAD. Sebaliknya, pajanan timbal dapat menghambat proses konversi 25(OH)D, menyebabkan penurunan kadar vitamin D. Tujuan dari penelitian ini adalah untuk mengetahui korelasi antara kadar 25(OH)D serum dan kadar timbal darah dalam kaitannya dengan aktivitas Delta-ALAD. Bahan dan Metode Dalam penelitian potong lintang ini, kami meneliti 90 pekerja laki-laki yang terpajan timbal sebagai bagian dari pekerjaan atau lingkungan tempat tinggal mereka di Jawa, Indonesia. Kami menilai aktivitas 25(OH)D, kadar timbal darah, dan Delta-ALAD dalam serum di laboratorium, dengan menggunakan Electrochemiluminescense Immunoassay (ECLIA) untuk 25(OH)D, ICP-MS untuk kadar timbal darah, dan spektrofotometri untuk Delta-ALAD. Analisis data dilakukan dengan uji Spearman untuk mengetahui korelasi antara serum 25(OH)D, kadar timbal darah, dan aktivitas Delta-ALAD. Hasil Kami menemukan korelasi negatif yang signifikan antara kadar timbal darah dan aktivitas Delta-ALAD (koefisien korelasi r = -0,39, p < 0,001), dan korelasi positif antara rasio 25(OH)D dan kadar timbal darah terhadap aktivitas Delta-ALAD (koefisien korelasi r = 0,28, p = 0,007). Namun, tidak ada korelasi yang diamati antara kadar 25(OH)D serum dan aktivitas Delta-ALAD. Kesimpulan Kadar timbal darah yang lebih tinggi berkorelasi dengan aktivitas Delta-ALAD yang lebih rendah. Hasil penelitian kami juga menyoroti peran potensial vitamin D sebagai antioksidan untuk menghambat efek timbal pada sintesis heme. Perlu dilakukan investigasi lebih lanjut untuk menentukan mekanismenya.
Kata kunci: Delta Aminolevulinic Acid Dehydratase, Kadar timbal darah, Pajanan timbal, Pekerja, 25(OH)D


Objectives Lead disrupts the heme synthesis pathway by modifying the activity of DeltaAminolevulinic Acid Dehydratase (ALAD), leadig to oxidative stress. In this mechanism, vitamin D acting as an antioxidant, might play a role in regulating the changes in ALAD activity. Conversely, lead exposure can impede the conversion process of 25(OH)D, causing a reduction in vitamin D levels. The objective of this study was to investigate the correlation between serum 25(OH)D levels and blood lead levels (BLL) in relation to Delta-ALAD activity. Material and Methods In this cross-sectional study, we examined 90 male workers who were exposed to lead as part of either their occupation or their living environment in Java, Indonesia. We assessed serum 25(OH)D, BLL, and Delta-ALAD activity in a laboratory setting, employing Electrochemiluminescense Immunoassay (ECLIA) for 25(OH)D, ICP-MS for BLL, and spectrophotometry for Delta-ALAD. Data analysis was carried out with Spearman test to find out the correlation between serum 25(OH)D, blood lead level and DeltaAminolevulinic Acid Dehydratase activity. Results We found a significant negative correlation between blood lead levels (BLL) and ALAD activity (correlation coefficient r=-0.39, p < 0.001), and a positive correlation between the ratio of 25(OH)D to BLL and ALAD activity (correlation coefficient r=0.28, p=0.007). However, no correlation was observed between the levels of serum 25(OH)D and DeltaALAD activity. Conclutions Higher BLL was correlated to lower Delta-ALAD activity. Our results also highlighted the potential role of vitamin D as an antioxidant to inhibit the effect of lead on heme synthesis. It is necessary to conduct further investigation to determine its mechanism.
Keywords: Blood lead levels, Delta Aminolevulinic Acid Dehydratase, Lead exposure, 25(OH)D, Worker