Tesis

Determining Effect of Metamizole to Reduce Risk of Renal Ischemic Reperfusion Injury Based on IL-18 Level = Analisis Pengaruh Metamizole dalam Menurunkan Risiko Cedera Reperfusi Iskemik Ginjal melalui Pengukuran Kadar Interleukin- 18 (IL-18).

Objective : This study aims to evaluate the effect of metamizole administration on IL-18 levels in a rat model of RIRI. Methods: This experimental study involved 32 adult male Wistar rats, divided into four groups: negative control, positive control, metamizole 100 mg/kg, and metamizole 200 mg/kg. RIRI was induced by bilateral renal pedicle clamping for 45 minutes. IL-18 levels were measured using ELISA. Results: ANOVA analysis revealed a significant difference in IL-18 levels between groups (p < 0.001). The 100 mg/kg metamizole group had the lowest IL-18 level, while the 200 mg/kg dose was associated with significantly elevated IL-18 levels compared to other groups, indicating potential toxicity at higher doses. Conclusion: Metamizole at 100 mg/kg may reduce IL-18 levels and mitigate the risk of renal ischemia-reperfusion injury. However, caution is advised with higher doses, as they may induce nephrotoxicity. Further research is necessary to determine the optimal therapeutic dose.
Keywords: Renal Ischemia-Reperfusion Injury (RIRI), Metamizole, Interleukin-18 (IL-18), Acute Kidney Injury (AKI)


Objektif: Studi ini bertujuan untuk menilai efek pemberian metamizole terhadap kadar IL-18 dalam model hewan RIRI. Metode: pStudi eksperimental ini dilakukan pada 32 tikus Wistar jantan dewasa yang dibagi menjadi empat kelompok: kontrol negatif, kontrol positif, metamizole dosis 100 mg/kg, dan 200 mg/kg. Induksi RIRI dilakukan melalui penjepitan pedikel ginjal bilateral selama 45 menit. Kadar IL-18 diukur menggunakan metode ELISA. Hasil: Analisis ANOVA menunjukkan perbedaan bermakna kadar IL-18 antar kelompok (p < 0.001). Kelompok metamizole 100 mg/kg memiliki kadar IL-18 terendah, sedangkan pemberian dosis 200 mg/kg menghasilkan peningkatan kadar IL-18 yang signifikan dibanding kelompok lain, menunjukkan potensi toksisitas pada dosis tinggi. Kesimpulan: Pemberian metamizole dosis 100 mg/kg menunjukkan potensi dalam menurunkan kadar IL-18 dan kemungkinan mengurangi risiko cedera reperfusi ginjal. Namun, dosis tinggi (200 mg/kg) dapat berdampak merugikan. Diperlukan studi lanjutan untuk menentukan dosis optimal dan keamanannya.
Kata Kunci: Cedera Iskemik Reperfusi Ginjal (RIRI), Metamizole, Interleukin-18 (IL-18), Cedera Ginjal Akut (AKI)