Tesis

Efek Fraksi Etanol Centella asiatica dalam Mencegah Resistensi Insulin pada Mencit yang Diinduksi Diet Tinggi Lemak dan Fruktosa terhadap Proses Inflamasi dan Penuaan Sel = The Effect of Centella asiatica Ethanolic Fraction on Insulin Resistance Prevention in Mice Induced by High Fat Diet and Fructose on Inflammation and Senescence Process.

Obesitas merupakan kondisi metabolik kronis yang menjadi faktor risiko dalam perkembangan resistensi insulin atau prediabetes. Agen antiinflamasi memiliki potensi menghambat terjadinya resistensi insulin dan senescence sehingga bisa mengurangi progresivitas ke arah penyakit metabolik. Diperlukan investigasi agen anti-inflamasi baru yang memiliki efikasi yang lebih baik dengan efek samping minimal. Penelitian ini dibuat untuk menganalisis efek fraksi etanol Centella asiatica terhadap resistensi insulin. Penelitian eksperimental dengan sampel mencit galur DDY, terdiri dari empat kelompok penelitian, yaitu kelompok normal, kelompok HFD, kelompok CA 150, dan kelompok CA 300. Model obesitas dilakukan dengan diet tinggi lemak (HFD) dan pemberian cairan fruktosa 25%. Dilakukan uji insulin tolerance test (ITT) pada minggu ke-13 dan glucose tolerance test (GTT) pada minggu ke-14. Pemeriksaan dilakukan untuk kadar trigliserida darah, pewarnaan SA-β-galactosidase pada jaringan adiposa, serta ekspresi protein AKT, pAKT, mRNA IRS-1, IL-6, IL-1β , P21, P16 pada jaringan adiposa. Hasil analisis memperlihatkan bahwa pemberian Centella asiatica dosis 150 dan 300mg/kgBB menurunkan berat badan, kadar trigliserida dan kadar glukosa darah, selain itu dapat meningkatkan ekspresi relatif mRNA IRS-1, p16, dan p21, menurunkan ekspresi relatif mRNA IL-1Β , IL6, serta menurunkan intensitas warna biru pada pewarnaan SA-β-galactosidase WAT. Tapi, pengukuran dengan menggunakan Western Blot, Centella asiatica dosis 150 dan 300mg/kgBB tidak mampu meningkatkan intensitas pita pAKT. Temuan-temuan ini membuktikan fraksi etanol Centella asiatica memiliki potensi dalam mencegah resistensi insulin melalui jalur inflamasi dan senescence.
Kata kunci: Resisrtensi insulin, Centella asiatica, High fat diet, Senescence


Obesity is a chronic metabolic condition that is a risk factor in the development of insulin resistance or prediabetes. Anti-inflammatory agents have the potential to inhibit the occurrence of insulin resistance and senescence so that they can reduce the progression towards metabolic disease. Investigation of new anti-inflammatory agents that have better efficacy with minimal side effects is needed. This research was conducted to analyze the effect of Centella asiatica water extract on insulin resistance. Experimental research with samples of DDY strain mice, consisting of four research groups, namely the normal group, HFD group, CA 150 group, and CA 300 group. The obesity model was carried out using a high-fat diet (HFD) and administration of 25% fructose fluids. An insulin tolerance test (ITT) was carried out at the 13th week and a glucose tolerance test (GTT) at the 14th week. Examinations were carried out for blood triglyceride levels, SA-β-galactosidase staining of adipose tissue, as well as protein expression of AKT, pAKT, IRS-1, IL- 6, IL-1β , P21, P16 mRNA in adipose tissue. The results of the analysis showed that administration ofCentella asiatica at doses of 150 and 300 mg/kgBW reduced body weight, triglyceride levels and blood glucose levels, in addition to increasing the relative expression of IRS-1, p16, and p21 mRNA, reducing the relative expression of IL-1Β , IL6, and mRNA. As well as reducing the intensity of the blue color in SA-β-galactosidase WAT staining. However, measurements using Western Blot, Centella asiatica doses of 150 and 300mg/kgBW were not able to increase the intensity of the pAKT band. These findings prove that Centella asiatica water extract has the potential to prevent insulin resistance through inflammation and senescence pathways.
Keywords: Insulin resistance, Centella asiatica, High fat diet, Senescence