Tesis

Pengaruh Sel Punca Mesenkim Tali Pusat terhadap kadar VEGF pada tikus cedera hati kronis serta ekspresi VEGFR2 dan ETBR Sinusoid = The Influence of Umbilical Cord-Derived Mesenchymal Stem Cells on the VEGF level in chronic liver injury rat and the expression of Sinusoidal VEGFR2 and ETBR.

Cedera hati kronis ditandai adanya aktivasi hepatic stellate cells (HSC) dan aktivasi sel endotel sinusoid. HSC yang aktif merubah konformasi miofibroblas, sedangkan aktivasi sel endotel memicu ketidakseimbangan vasokontriksi dan vasodilatasi. Pengamatan lebih lanjut pada vascular endothelial growth factor (VEGF) di jaringan hati dan peran sel endotel sinusoid melalui vascular endothelial growth factor receptor 2 (VEGFR2) dan endothelin B receptor (ETBR). Penelitian ini bertujuan untuk menganalisis pengaruh pemberian terapi sel punca mesenkim asal tali pusat (SPMTP) dosis 1 juta dan 3 juta sel pada 2-acetylaminoflurine (2AAF) dan carbon tetrachloride (CCl4). Penelitian ini merupakan penelitian eksperimental dari bahan biologis tersimpan berupa jaringan hati tikus (Rattus novergivus) strain Wistar Jantan usia 8 minggu, terdiri dari kelompok 2AAF/CCl4+1 juta SPM-TP, 2AAF/CCl4 + 1 juta SPM-TP, 2AAF/CCl4 dan sehat. Metode Enzyme-Linked Immunosorbent Assay (ELISA) digunakan untuk analisis kadar VEGF di jaringan hati. Hasil uji one way ANOVA menunjukkan perbedaan bermakna (p < 0,014), analisis Gomes howell terlihat perbedaan bermakna kelompok 2AAF/CCI4 + 1 juta SPM-TP dengan 2AAF/CCl4 (p < 0.048) dan kelompok 2AAF/CCI4+3 juta SPM-TP dengan 2AAF/CCl4 (p < 0.002). Hasil analisis VEGFR2 menggunakan uji one way ANOVA menunjukkan tidak berbeda bermakna antar kelompok (p > 0.089). Hasil analisis ETBR menggunakan uji one way ANOVA menunjukkan adanya perbedaan bermakna (p < 0,011), analisis post hoc Bonferroni terlihat perbedaan bermakna kelompok 2AAF/CCI4+1 juta SPM-TP dengan 2AAF/CCl4 (p < 0.031), dan kelompok 2AAF/CCl4+3 juta SPM-TP dengan kelompok 2AAF/CCl4 (p < 0.038). Hasil analisis korelasi menunjukkan korelasi tidak bermakna antara VEGF dengan ETBR (p < 0.321).
Kata kunci: Sel endotel sinusoid, VEGF, VEGFR2, ETBR, SPM-TP


Chronic liver injury is characterized by the activation of Hepatic Stellate Cells (HSC) and sinusoidal endothelial cell activation. Active HSCs alter the conformation of myofibroblasts, while endothelial cell activation triggers an imbalance between vasoconstriction and vasodilation. Further observations focus on Vascular Endothelial Growth Factor (VEGF) in liver tissue and the role of sinusoidal endothelial cells through Vascular Endothelial Growth Factor Receptor 2 (VEGFR2) and endothelin B receptor (ETBR). This study aims to analyze the influence of Umbilical chord-derived Mesenchymal Stem Cell therapy (UC-MSC) at doses of 1 million and 3 million cells on 2-Acetylaminofluorine (2AAF) and Carbon Tetrachloride (CCl4)-induced liver injury. The experimental research utilized stored biological materials in the form of liver tissues from 8-week-old male Wistar rats, consisting of groups: 2AAF/CCl4+1 million UC-MSC, 2AAF/CCl4+3 million UC-MSC, 2AAF/CCl4, and a healthy control. Enzyme-Linked Immunosorbent Assay (ELISA) was used for VEGF analysis in liver tissues. One-way ANOVA results showed significant differences (p < 0.014), with post hoc Bonferroni analysis revealing significant differences between 2AAF/CCl4+1 million UC-MSC and 2AAF/CCl4 (p < 0.048) and between 2AAF/CCl4+3 million UC-MSC and 2AAF/CCl4 (p < 0.002). VEGFR2 analysis showed no significant differences among groups (p > 0.089). ETBR analysis using one-way ANOVA indicated significant differences (p < 0.011), and Bonferroni post hoc analysis revealed significant differences between 2AAF/CCl4+1 million UC-MSC and 2AAF/CCl4 (p < 0.031), and between 2AAF/CCl4+3 million UC-MSC and 2AAF/CCl4 (p < 0.038). Correlation analysis indicated no significant correlation between VEGF and ETBR (p < 0.321).
Keywords: Sinusoidal endothelial cells, VEGF, VEGFR2, ETBR, UC-MSCs