Tesis

Efek Hibiscus sabdariffa Linn. terhadap kadar FGF21 dan AMPK pada Jantung Mencit Diabetes Melitus = The effects ofHibiscus sabdariffa Linn. on the levels of FGF21 and AMPK in the Heart of Diabetic Mice.

Diabetes Melitus (DM) merupakan gangguan metabolisme yang ditandai dengan disfungsi sel pankreas dan resistensi insulin. Fibroblast Growth Factor 21 (FGF21) dianggap memiliki peran kardioprotektif. Melalui pensinyalan FGF21, aktivitas AMPactivated Protein Kinase (AMPK) berperan mengurangi apoptosis, peradangan, hipertrofi, dan fibrosis pada jantung. Hibiscus sabdariffa Linn. (HSL) mengandung flavonoid yang memberikan efek hipoglikemik bersamaan dengan aktivasi antiinflamasi dan antioksidan. Penelitian ini bertujuan menilai efek HSL terhadap kadar FGF21 dan AMPK pada jantung model mencit diabetes. Penelitian eksperimental menggunakan mencit Deutschland Denken Yoken (DDY) usia 8 minggu, dibagi ke dalam 4 kelompok yang berbeda: Kontrol, DM Kontrol, HSL 200, dan HSL 400. Pada kelompok DM kontrol, HSL 200 dan HSL 400 dilakukan induksi diabetes dengan pemberian High Fat Diet (HFD) dan STZ 40mg/kgbb. Kelompok HSL 200 diberikan suplementasi HSL 200mg/kgBB selama 3 minggu dan kelompok HSL 400 diberikan suplementasi HSL 400 mg/kgBB selama 3 minggu. Kadar FGF21 dan AMPK diukur dengan metode enzyme-linked immunosorbent assay (ELISA). Tidak terdapat perbedaan yang bermakna antara kadar FGF 21 dan AMPK antara kelompok kontrol dan DM kontrol. Kadar FGF21 dan AMPK pada kelompok HSL 400 lebih tinggi dibandingkan kelompok Kontrol, DM Kontrol, dan HSL 200. Temuan ini menunjukkan bahwa suplementasi HSL pada dosis tertentu berpotensi meningkatkan aktivasi pensinyalan FGF 21 dan AMPK pada jantung model mencit diabetes.
KATA KUNCI : FGF21; AMPK; Diabetes Melitus; Hibiscus sabdariffa Linn.


Diabetes Mellitus (DM) is a metabolic disorder characterized by pancreatic cell dysfunction and insulin resistance. Fibroblast Growth Factor 21 (FGF2) is thought to have a cardioprotective role. Through FGF21 signaling, AMP-activated Protein Kinase (AMPK) activity plays a role in reducing apoptosis, inflammation, hypertrophy and fibrosis in the heart. Hibiscus sabdariffa Linn. (HSL) contains flavonoids which provides a hypoglycemic effect and anti inflammatory and antioxidant activation. act as antidiabetics. This study aims to analyse the effect of HSL on FGF21 and AMPK levels in the heart of diabetic mice model. Experimental research using Deutschland Denken Yoken (DDY) mice aged 8 weeks, randomly divided into 4 different groups: Control, DM Control, HSL 200, and HSL 400. Diabetes were induced to DM control group, HSL 200 group and HSL 400 group by administering High Fat Diet (HFD) and STZ 40mg/kgbb . The HSL 200 group was given HSL 200mg/kgBW supplementation for 3 weeks and the HSL 400 group was given HSL 400 mg/kgBW supplementation for 3 weeks. FGF21 and AMPK levels were measured using the enzyme-linked immunosorbent assay (ELISA) method. There was no significant difference ofFGF 21 and AMPK levels between the control and DM control groups. The levels of FGF21 and AMPK in the HSL 400 group were higher than those in the Control, DM Control, and HSL 200 groups. These findings indicate that HSL supplementation at certain doses has the potential to increase the activation of FGF 21 and AMPK signaling in the hearts of diabetic mice models. \
KEYWORD : FGF21; AMPK; Diabetes Melitus; Hibiscus sabdariffa Linn.