Tesis

Analisa Respon Makrofag terhadap LDL (Low Density Lipoprotein) Teroksidasi pada Sindrom Ovarium Polikistik Obesitas dan nir-Obesitas = Analysis of Macrophage Response to Oxidized LDL (Low Density Lipoprotein) in Obese and Lean Polycystic Ovary Syndrome.

Latar Belakang Sindrom ovarium polikistik (SOPK) merupakan salah satu penyakit infertilitas wanita yang ditandai dengan peradangan kronis tingkat rendah. Kondisi peradangan pada SOPK masih bertentangan karena seringkali tidak terdeteksi dalam sistem peredaran darah. Namun, diketahui bahwa monosit dan makrofag wanita SOPK lebih rentan terhadap peradangan dibandingkan dengan yang sehat. Penelitian ini bertujuan untuk mengevaluasi respon makrofag terhadap paparan ox-LDL secara in vitro dalam mengekspresikan CD36 dan sitokin tertentu. Metode 20 wanita SOPK obesitas, 20 wanita SOPK nir-Obesitas, dan 18 wanita sehat sebagai kelompok kontrol direkrut dalam penelitian ini. Data antropometri diukur dan darah tepi dikumpulkan. Sel mononuklear darah diisolasi dan kemudian dikultur. Monosit dipilih melalui adhesi dan didiferensiasi menjadi makrofag. Makrofag kemudian dipajan dengan ox-LDL selama 24 jam dan melalui flow cytometri untuk memeriksa ekspresi CD36, TNF-α, IL-1Β, dan IL-10. Hasil Pola ekspresi CD36 ditemukan secara statistik lebih tinggi pada SOPK Obesitas. TNF-α dan IL-10 juga secara signifikan lebih tinggi pada SOPK Obesitas dibandingkan dengan SOPK Nir-Obesitas dan kontrol. Korelasi positif hanya ditemukan antara CD36 dan IL-10. Kesimpulan Makrofag wanita SOPK Obesitas merespons ox-LDL dengan cara yang berbeda dibandingkan dengan SOPK Lean dan kelompok kontrol dengan pola ekspresi CD36, TNF-α, dan IL-10 yang lebih tinggi.
Kata Kunci: CD36, IL-1Β, IL-10, makrofag, SOPK, and TNF-α.


Introduction Polycystic ovary syndrome (PCOS) is one of the female infertility diseases that is signed with low-grade chronic inflammation. The inflammation condition in PCOS is conflicting as it was often unable to be detected in the circulating blood system. However, it is known that monocyte of PCOS patients were more susceptible to inflammation compared to healthy ones. This research aimed to evaluate in vitro macrophage response to ox-LDL exposure in expressing CD36 and certain cytokines. Method 20 Obese PCOS, 20 Lean PCOS, and 18 healthy women as the control group were recruited in this study. Anthropometrics data were measured and peripheral blood was collected. Blood mononuclear cells were isolated and then cultured. Monocytes were selected by adhesion and differentiated into macrophages. Macrophages were then exposed to oxidized Low Density Lipoprotein (ox-LDL) for 24 hours and underwent flow cytometry to examine the expression of CD36, TNF- a, IL-1Β, and IL-10. Results CD36 expression pattern was found to be statistically higher in Obese PCOS. TNF- α and IL-10 were also significantly higher in Obese PCOS compared to Lean PCOS and control. Positive correlation was only found between CD36 and IL-10. Conclusion Macrophage of Obese PCOS women responded to ox-LDL in different way compared to Lean PCOS and control group with higher expression pattern ofCD36, TNF-α, and IL-10.
Keywords: CD36, IL-1Β, IL-10, makrofag, PCOS, and TNF-α.