Tesis

Efek Kombinasi Topikal dan Oral Ekstrak Etanol Daun Ungu (Graptophyllum pictum) terhadap Tingkat Inflamasi Jaringan Anorektal Tikus Hemoroid = The Effect of Topical and Oral Combination of Ethanolic Extract of Graptophyllum pictum Leaf on the Inflammation Level of Anorectal Tissue in Hemorrhoid Rat.

Latar belakang: Hemoroid merupakan suatu kondisi patologis yang ditandai dengan adanya pelebaran pembuluh darah abnormal dan pembengkakan pada anorektal yang disertai inflamasi. Penelitian ini bertujuan untuk menganalisis potensi ektrak daun ungu yang diberikan secara topikal dan oral dalam meregulasi inflamasi pada jaringan anorektal tikus Hemoroid yang diinduksi croton oil 10%. Metode: Hewan coba diinduksi Hemoroid menggunakan croton oil 10% selama 3 hari kemudian dilanjutkan terapi sesuai kelompok selama 7 hari. Setelah terminasi, organ anorektal diisolasi dan dibagi menjadi 2 untuk pembuatan preparat dan pengukuran parameter imunologi. Level sitokin IL-6 dan TNF-α diukur menggunakan ELISA sedangkan mRNA iNOS dan IL-10 diukur dengan qRT-PCR. Pengamatan histopatologi yang dilakukan meliputi pengukuran diameter vena, skoring nekrosis, dan perhitungan koefisien anorektal. Hasil: Kombinasi topikal dan oral ekstrak daun ungu cenderung menurunkan sitokin IL-6 dan TNF-α, menurunkan ekspresi mRNA iNOS, dan menurunkan dilatasi pembuluh darah meski tidak signifikan secara statistik, menurunkan skoring nekrosis secara signifikan, serta meningkatkan ekspresi mRNA IL-10 secara signifikan. Namun, ekstrak daun ungu yang diberikan secara topikal dan oral tidak tampak menurunkan koefisien anorektal tikus. Kandungan berbagai senyawa aktif pada ekstrak daun ungu dipercaya dapat meregulasi inflamasi jaringan dan mempromosikan penyembuhan jaringan
Kata kunci: Daun ungu, Flavonoid, Inflamasi, Hemoroid


Background: Hemorrhoids are a pathological condition characterized by abnormal blood vessel dilation and anorectal swelling accompanied by inflammation. This study aims to analyze the potential of purple leaf extract given topically and orally in regulating inflammation in the anorectal tissue of hemorrhoid rats induced by 10% croton oil. Methods: Hemorrhoids were induced using 10% Croton oil for 3 days then continued with therapy according to each group for 7 days. After termination, the anorectal organ was isolated and divided into 2 parts, for histopathological analysis and measurement of immunological parameters. IL-6 and TNF-α cytokine levels were measured using ELISA while iNOS and IL-10 mRNA were measured using qRTPCR. Histopathological observations included measuring vein diameter, necrotizing scoring, and calculating the anorectal coefficient. Result: The topical and oral combination of purple leaf extract tends to reduce the cytokines IL-6 and TNF-α, decrease iNOS mRNA expression, and reduce blood vessel dilatation although not statistically significant, reduce necrosis scoring significantly, and increase IL-10 mRNA expression significantly. However, purple leaf extract administered topically and orally did not appear to reduce the anorectal coefficient. The content of various active compounds in purple leaf extract is believed to regulate tissue inflammation and promote tissue healing
Keywords: Purple Leaf, Inflammation, Flavonoid, Hemorrhoids