Tesis

Studi In Vitro Efek Ekstrak Etanol Gambir (Uncaria gambir (Hunter) Roxb.) terhadap Ekspresi Sitoglobin, Elastin, dan Desmosin pada Fibroblas Keloid = In Vitro Study on The Effect of Ethanol Extract of Gambir (Uncaria gambir (Hunter) Roxb.) on The Expression of Cytoglobin, Elastin, and Desmosine in Keloid Fibroblast.

Keloid merupakan jaringan tumor jinak yang tumbuh akibat proses penyembuhan luka menyimpang. Keloid dapat menimbulkan masalah kosmetik maupun gangguan fungsi tubuh, terutama bila keloid timbul pada daerah persendian. Patogenesisnya belum sepenuhnya diketahui, namun fibroblas teraktivasi terlibat dalam dekomposisi kolagen berlebih pada matriks ekstraseluler. Di sisi lain, fibroblas mensintesis sitoglobin. Ekspresi sitoglobin berlebih dapat menekan proliferasi fibroblas. Berbagai terapi keloid hingga saat ini belum optimal karena masih sering terjadi rekurensi. Oleh karena itu, berbagai penelitian untuk mencari terapi keloid masih terus berkembang. Pada penelitian ini digunakan ekstrak etanol gambir (Uncaria gambir (Hunter) Roxb.) untuk mengeksplorasi pengaruh ekstrak etanol gambir (EG) terhadap viabilitas fibroblas keloid serta ekspresi sitoglobin, elastin dan desmosin. Penelitian dilakukan dengan isolasi dan kultur primer fibroblas keloid serta dianalisis populasi fibroblas, nilai IC50 EG terhadap fibroblas keloid, serta ekspresi sitoglobin, elastin dan desmosin. Didapatkan hasil bahwa EG dapat menurunkan viabilitas fibroblas keloid dan signifikan meningkatkan ekspresi sitoglobin, elastin dan desmosin. Terdapat korelasi positif antara penurunan viabilitas fibroblas keloid dengan ekspresi protein sitoglobin, elastin dan desmosin, namun tidak signifikan. Juga, terdapat korelasi positif signifikan antara ekspresi sitoglobin dengan elastin dan desmosin. Selain itu, terdapat korelasi positif signifikan antara ekspresi elastin dengan desmosin. Dengan demikian, EG berpotensi sebagai antikeloid.
Kata Kunci: Fibroblas keloid, ekstrak etanol gambir, sitoglobin, elastin, desmosin.


Keloids are benign tumor that grow as a result of aberrant wound healing processes. Keloids can cause cosmetic problems and impaired body function, especially if they occur in the joint area. The pathogenesis isn’t fully understood, but activated fibroblasts are involved in the decomposition of excess collagen in the extracellular matrix. In contrast, fibroblasts synthesize cytoglobin. Cytoglobin overexpression can suppress fibroblast proliferation. Various keloid therapies until now haven’t been optimal due to numerous recurrences. Therefore, various studies to find keloid therapy are still developing. In this study, ethanol extract of gambir (Uncaria gambir (Hunter) Roxb.) was used to explore the effect of ethanolic extract of gambir (EG) on the viability of keloid fibroblasts and the expression of cytoglobin, elastin, and desmosine. The experiment done by isolation and primary culture of keloid fibroblasts and analyzed the fibroblast population, IC50 EG value of keloid fibroblasts, and cytoglobin, elastin, and desmosine expression. The result showed that EG can decrease the viability of keloid fibroblasts and significantly increase the expression of cytoglobin, elastin, and desmosine. There is a positive correlation between the decrease in keloid fibroblast viability and the expression of cytoglobin, elastin, and desmosine proteins, but it is not significant. Also, a significant positive correlation between cytoglobin expression with elastin and desmosine. Furthermore, there is a significant positive correlation between the expression of elastin and desmosine. Thus, EG has the potential as an anti-keloid.
Keywords: Keloid fibroblast, ethanol extract of gambir, cytoglobin, elastin, desmosine