Tesis

Pengaruh Ekstrak Etanol Uncaria Gambir (Hunter) Roxb. terhadap viabilitas galur sel HepG2 dan korelasinya terhadap ekspresi Sitoglobin, Nrf2 dan PDGFA = Effect of Gambir (Uncaria gambir (Hunter)Roxb.) Ethanol Extract on Proliferation of Hep G2 : Focus on Expression of Cytoglobin, Nrf2, and PDGFA.

Karsinoma hepatoseluler (HCC) merupakan masalah kesehatan global dengan tatalaksana yang belum memberikan hasil optimal. Gambir adalah tanaman spesifik Indonesia, yang mengandung flavonoid, dan digunakan sebagai antikanker. Pada patogenesis HCC terjadi disregulasi CYGB, Nrf2, dan PDGFA. Jusman et al mendapatkan bahwa senyawa dalam gambir dapat berikatan dengan PDGFA. Penelitian ini digunakan ekstrak etanol gambir (EG), dan bertujuan untuk menilai pengaruh EG terhadap viabilitas galur sel HepG2, dan korelasinya terhadap ekspresi CYGB, Nrf2, dan PDGFA. Optimasi konsentrasi DMSO sebagai pelarut EG, dan konsentrasi EG pada HepG2 (IC50) dianalisis dengan uji MTT. Sel HepG2 diisolasi proteinnya dan ekspresi Nrf2, dan PDGFA menggunakan ELISA, serta CYGB menggunakan ELISA dan Imunofluoresens. Analisis proliferasi sel HepG2 dengan uji BrdU. Hasil dari analisis viabilitas, proliferasi, dan ELISA dari kelompok kontrol dan perlakuan dibandingkan secara statistik. Kami juga menganalisis korelasi antara masing-masing variabel. Hasil menunjukan pemberian EG konsentrasi 500; 1000; dan 2000 μg/mL menyebabkan penurunan viabilitas sel, proliferasi sel, serta penurunan ekspresi protein CYGB, Nrf2, dan PDGFA sebanding dengan peningkatan pemberian konsentrasi EG. Terdapat korelasi positif dan bermakna antara viabilitas dengan proliferasi sel HepG2. Terdapat korelasi positif dan bermakna antara viabilitas sel HepG2 dengan ekpresi protein CYGB, Nrf2, dan PDGFA. Selain itu, terdapat korelasi positif dan bermakna antara ekspresi CYGB dengan Nrf2, dan protein Nrf2 dengan PDGFA, dan protein CYGB dengan PDGFA. Disimpulkan pemberian EG menurunkan viabilitas, dan proliferasi sel HepG2 serta terbukti menurunkan ekspresi CYGB, Nrf2, dan PDGFA.
Kata kunci : HepG2, ektrak etanol gambir, sitoglobin, Nrf2, PDGFA.


Hepatocellular carcinoma (HCC) is a global health problem with treatment has not provided optimal results. Gambir is an Indonesian specific plant, which contains flavonoids, and is used as an anticancer. Gambir is an Indonesian specific plant, which contains flavonoids, and is used as an anticancer. In the pathogenesis of HCC dysregulation of Nrf2, CYGB, and PDGFA occurs. Jusman et al found that compounds in gambir can bind to PDGFA. This study used gambier ethanol extract (EG), and aimed to assess the effect of EG on HepG2 cell proliferation, as well as the expression of CYGB, Nrf2, and PDGFA. Optimization of DMSO concentration as EG solvent, and EG concentration in HepG2 (IC50) were analyzed by MTT test. Protein of HepG2 were isolated and expression of Nrf2 and PDGFA used ELISA, and CYGB used ELISA and Immunofluorescence. Analysis of HepG2 cell proliferation with BrdU assay. Results from viability, proliferation, and ELISA analyses of the control and treatment groups were compared statistically. We also analyzed the correlation between each variable. The results showed the administration of EG concentration 500; 1000; and 2000 μg/mL led to decreased cell viability, cell proliferation, and decreased expression of CYGB, Nrf2, and PDGFA proteins comparable to an increase in EG concentration. There is a positive and significant correlation between viability and proliferation of HepG2 cell. There is a positive and significant correlation between HepG2 cell viability and CYGB, Nrf2, and PDGFA protein expression. In addition, there is a positive and significant correlation between the expression of CYGB and Nrf2, the protein Nrf2 and PDGFA, and the expression of CYGB with PDGFA. It was concluded that EG administration decreased the viability, and proliferation of HepG2 cells and was shown to decrease the expression of CYGB, Nrf2, and PDGFA.
Keywords : HepG2, gambir ethanol extract, cytoglobin, Nrf2, PDGFA.