Tesis

Hubungan Antara Ekspresi Biomarker Matrix Metalloproteinase- 9 (MMP-9) pada Meningioma dengan Infiltrasi Sel Tumor ke Tulang pada Pasien Meningioma di RSUPN Dr. Cipto Mangunkusumo = The Role of Matrix Metalloproteinase-9 In Meningioma Infiltration to The Bone.

Pendahuluan Matrix Metalloproteinase-9 diyakini berperan dalam infiltrasi meningioma ke tulang yang menyebabkan hiperostosis. Studi prospektif ini diharapkan dapat mempelajari tentang MMP-9 dan hubungannya dengan sifat infiltratif meningioma Metode Analisis prospektif dilakukan untuk mengambil sampel meningioma bersama dengan tulang yang berdekatan melalui operasi. Pewarnaan hematoxylin dan eosin dilakukan untuk mengidentifikasi infiltrasi tumor ke kalvaria dari spesimen tulang yang berdekatan dengan tumor. Uji imunohistokimia sampel tumor dilakukan untuk menentukan intensitas MMP-9, yang diklasifikasikan menjadi empat kategori: negatif, lemah, sedang, dan kuat. Hasil Tiga puluh kasus meningioma menjalani operasi pengangkatan tumor. Terdapat 18 (60%) sampel dengan hiperostosis, dan 17 (94,4%) diantaranya menginfiltrasi tengkorak. Tidak ada sampel dengan pewarnaan imunohistokimia negatif. Enam belas (59,26%) sampel tumor memiliki intensitas ekspresi MMP-9 sedang, ekspresi lemah ditemukan pada tiga sampel (11,11%), sedangkan intensitas kuat ditemukan pada delapan kasus (29,63%). Hasil kami menunjukkan hubungan yang signifikan antara infiltrasi tumor ke tulang dengan hiperostosis, namun intensitas MMP-9 tidak berkorelasi signifikan dengan hiperostosis dan infiltrasi tumor. Kesimpulan Sebagai salah satu enzim proteolitik, kami menemukan bahwa MMP-9 bukanlah faktor yang signifikan untuk proses infiltrasi meningioma ke tulang kepala.
Kata kunci: meningioma, hiperostosis, MMP-9, infiltrasi tumor



Introduction Matrix Metalloproteinase-9 is believed to play a role in meningioma infiltration to the bone that causes hyperostosis. This prospective study is expected to learn about MMP-9 and its relationship with the infiltrative nature of meningiomas Method A prospective analysis was conducted to retrieve meningioma samples along with adjacent bone through surgery. Hematoxylin and eosin staining was performed to identify tumoral infiltration to the calvaria from adjacent bone specimens. Immunohistochemical tests of tumor samples were conducted to determine MMP- 9 intensity, classified into four categories: negative, weak, moderate, and strong Results Thirty meningioma cases underwent craniotomy tumor removal. There were 18 (60%) samples with hyperostosis, and 17 (94.4%) of them infiltrated the skull. There was no sample with negative IHC staining. Sixteen (59.26%) tumor samples had moderate intensity expression of MMP-9, weak expression was found in three (11.11%) samples, while strong intensity was found in eight (29.63%) cases. Our result showed a significant relationship between tumor infiltration to the adjacent bone with hyperostosis, but the intensity of MMP-9 was not significantly correlated with hyperostosis and tumor infiltration. Conclusion As one of the proteolytic enzymes, we found that MMP-9 was not a significant factor for meningioma infiltration to the adjacent bone.
Keywords: meningioma, hyperostosis, MMP-9, tumor infiltration