Skripsi

Pengaruh Terapi Vitamin D3 terhadap Kidney Injury Molecule-1 (KIM-1) pada Tikus Model Prediabetes = The Effect of Vitamin D3 Therapy on Kidney Injury Molecule-1 (KIM-1) in Prediabetes Rats Model.

Latar belakang: Menurut hasil Riset Kesehatan Dasar yang dilakukan pada tahun 2018, prevalensi prediabetes adalah 57,1%. Prediabetes merupakan faktor risiko hiperfiltrasi ginjal yang dapat memicu kerusakan ginjal. Meskipun vitamin D umumnya tidak digunakan dalam tatalaksana hiperglikemia, efek anti-inflamasi, anti fibrotik dan mekanisme umpan balik pada sistem renin-angiotensin vitamin D dapat bermanfaat dalam tatalaksana prediabetes. Efek protektif terapi vitamin D terhadap kerusakan ginjal akut pada kondisi prediabetes perlu diteliti lebih lanjut. Metode: Penelitian ini adalah penelitian eksperimental pada model hewan yang menggunakan sampel urin tersimpan dari penelitian sebelumnya. Tikus dikelompokan dalam kelompok sehat, kelompok prediabetes, kelompok prediabetes dengan pemberian vitamin D 100 IU/kg BB/hari atau kelompok prediabetes dengan pemberian vitamin D 1000 IU/kg BB/hari. Sebanyak 6 ekor tikus dalam setiap kelompok diteliti selama 12 minggu. KIM-1 diukur dengan metode ELISA dan dianalisis menggunakan analisis uji One-way ANOVA. Hasil: Terdapat peningkatan kadar KIM-1 pada tikus prediabetes. Pemberian terapi vitamin D3 dosis 100 IU/kg BB/hari dan 1,000 IU/kg BB/hari menurunkan kadar KIM-1 jika dibandingkan dengan tikus model prediabetes yang tidak mendapatkan terapi vitamin D3. Namun, hanya vitamin D3 dosis 1,000 IU/kg BB/hari yang menurunkan kadar KIM- 1 pada tikus model prediabetes secara bermakna. Kesimpulan: Terapi vitamin D3 dosis 1,000 IU/kg BB/hari pada tikus model prediabetes menurunkan kadar KIM-1 secara bermakna (p = 0,043) jika dibandingkan dengan tikus model prediabetes yang tidak mendapatkan terapi vitamin D3.
Kata kunci: Vitamin D3, KIM-1, Prediabetes, Diabetes, Kerusakan ginjal akut



Introduction: According to the Riset Kesehatan Dasar in 2018, the prevalence of prediabetes was 57.1%. Prediabetes is a risk factor for renal hyperfiltration that can lead to kidney damage. Although vitamin D is not generally used to treat hyperglycemia, its anti-inflammatory effect, anti-fibrotic effect and feedback mechanisms on the reninangiotensin system may be useful in the management of prediabetes. Therefore, the protective effect of vitamin D therapy against acute kidney damage in prediabetes conditions is worthy of investigation. Method: This study is an experimental study using stored urine samples from a previous study. Rats were grouped into the healthy group, the prediabetes group, the prediabetes group which received 100 IU/kg BW/day of vitamin D or the prediabetes group which received 1000 IU/kg BW/day of vitamin D. Each group had 6 rats and were studied for 12 weeks. KIM-1 was measured by ELISA and analyzed using the One-way ANOVA test. Result: There was an increase in KIM-1 levels in prediabetic rats. Administration of vitamin D3 therapy at doses of 100 IU/kg BW/day and 1000 IU/kg BW/day reduced KIM- 1 levels when compared to prediabetic rats which did not receive vitamin D3 therapy. However, only 1000 IU/kg BW/day of vitamin D significantly reduced KIM-1 levels in prediabetic rats. Conclusion: Vitamin D3 therapy of 1000 IU/kg BW/day in prediabetic rats reduce the amount of KIM-1 significantly (p = 0.043) when compared to prediabetic rats which did not receive vitamin D3 therapy.
Keywords: Vitamin D3, KIM-1, Prediabetes, Diabetes, Acute kidney injury