Tesis

Ekspresi Immediate Early Response Gene X-1 (IEX-1) Saliva Sebagai Prediktor Keganasan Pada Tumor Ovarium Epitelial di RSUPN Dr. Cipto Mangunkusumo Jakarta = Salivary Immediate Early Response Gene X-1 (IEX-1) Expression as a Predictor of Malignancy in Epithelial Ovarian Tumors at RSUPN Dr. Cipto Mangunkusumo Jakarta.

Tesis ini bertujuan untuk menilai ekspresi Immediate Early Response Gene X-1 (IEX-1) saliva sebagai prediktor keganasan pada tumor ovarium epitelial. Merupakan penelitian uji diagnostik pada pasien tumor ovarium yang direncanakan operasi elektif dengan mengambil 3-5 ml saliva sebelum tindakan operasi. Dilakukan pemeriksaan ekspresi IEX-1 dengan Real Time qPCR. Dilakukan penilaian ekspresi IEX-1 pada tumor ovarium epitelial jinak dan ganas yang dikonfirmasi dengan pemeriksaan histopatologi jaringan tumor. Hasil Penelitian ini didapat dari 47 subjek. Rerata ekspresi IEX-1 saliva lebih tinggi pada tumor ovarium epitelial jinak (1,976) dibandingkan ganas (0,554) (p < 0,001). Didapatkan nilai AUC ekspresi IEX-1 0,949 (IK95% 0,894-1,000), nilai cut off point IEX-1 saliva ≥ 0.9115 dengan sensitivitas 84%, spesifisitas 86,4%, nilai duga positif 82,6% dan nilai duga negatif 87,5%. Terdapat hubungan yang signifikan antara ekspresi IEX-1 saliva dengan kejadian tumor ovarium epitelial ganas (OR 5,031, IK95% 2,03912,41; p < 0,001). Sebagai kesimpulan, terdapat hubungan yang bermakna antara penurunan ekspresi IEX-1 saliva dengan kejadian tumor ovarium epitelial ganas dengan sensitivitas dan spesifisitas yang cukup baik.
Kata Kunci: Ekspresi IEX-1, IEX-1 Saliva, Penanda Tumor, Tumor Ovarium Epitelial Ganas.


The diagnostic study aims to determine the expression of salivary IEX-1 as a predictor of malignancy in epithelial ovarian tumors. Patients with ovarian tumor who had been planned to undergo an elective surgery were taken as samples. The research material of this study is 3-5 ml of saliva which was taken before the surgery. IEX-1 expression was examined using the Real Time qPCR. We compared the salivary IEX-1 expression in benign and malignant epithelial ovarian tumors. This study was followed by 47 subjects, The mean salivary IEX-1 expression in benign epithelial ovarian tumors was higher (1.976) than the malignant tumors (0.554) (p < 0.001). The AUC value of IEX-1 expression was 0.949 (95% CI 0.894-1,000), cut off point of salivary IEX-1 is ≥ 0,9115 with sensitivity 84%, specificity 86,4%, positive predictive value 82.6% and negative predictive value 87.5%. There was a significant correlation between salivary IEX-1 expression and malignant epithelial ovarian tumors with an OR 5.031 (95% CI 2.03912.4; p < 0,001). In conclusion, there is a significant correlation between decreased salivary IEX-1 expression and the event of malignant epithelial ovarian tumors with a good sensitivity and specificity.
Keywords : Expression of IEX-1, Malignant Epithelial Ovarian Tumor, Salivary IEX-1, Tumor Marker.