Tesis

Perbandingan efek protektif elektroakupunktur dan akupunktur tanam benang terhadap tukak lambung pada tikus wistar = Comparison of the protective effects of electroacupuncture and thread implant acupuncture on gastric ulcers in wistar rats.

Pendahuluan: Tukak lambung merupakan salah satu penyakit tersering pada saluran pencernaan yang mempunyai angka kekambuhan yang cukup tinggi. Penanganan tukak lambung seringkali sulit dan membutuhkan biaya mahal. Terapi farmakologi memiliki banyak efek samping. Akupunktur sebagai salah satu terapi non-farmakologi telah menunjukkan hasil yang baik dalam terapi dan sebagai protektif terhadap tukak lambung. Tujuan penelitian ini adalah untuk mengetahui perbandingan efek protektif elektroakupunktur dengan akupunktur tanam benang terhadap indeks ulkus lambung dan kadar serum Malondialdehyde (MDA) pada tukak lambung. Metode: Penelitian dilakukan pada bulan November - Desember 2021 di Puslitbangkes Biomedik, Kementerian kesehatan Republik Indonesia, Salemba, Jakarta Pusat. Desain penelitian adalah studi eksperimental dengan Randomized posttest design. 30 hewan coba tikus dibagi menjadi 5 kelompok: kelompok normal, kontrol tukak lambung (TL), omeprazole (OME), elektroakupunktur (EA) dan akupunktur tanam benang (ATB). Kelompok OME diberikan omeprazole oral 20 mg/kg dan EA pada ST36 Zusanli dan CV12 Zhongwan dengan frekuensi 2 Hz, intervensi pada OME dan EA dilakukan setiap 2 hari sekali selama 12 hari. Kelompok ATB 1 kali intervensi di hari pertama. Skor indeks ulkus lambung dan kadar serum MDA diukur setelah induksi tukak lambung dilakukan pasca 12 hari perlakuan. Semua hasil data diolah menggunakan SPSS versi 20. Hasil: Skor indeks ulkus tidak berbeda bermakna antara kelompok EA dengan ATB (uji Mann Whitney, p = 0,523), namun skor indeks ulkus kelompok EA dan ATB lebih rendah bermakna dibandingkan kelompok TL (uji Mann Whitney, p < 0,05). Kadar serum MDA lebih rendah bermakna pada kelompok EA versus TL (uji post-hoc, p < 0,001) dan pada kelompok ATB versus TL (uji post-hoc, p < 0,05). Kelompok EA versus ATB, kadar MDA tidak berbeda bermakna (uji post-hoc, p = 1,000). Kesimpulan: Elektroakupunktur dan akupunktur tanam benang memiliki efek protektif terhadap tukak lambung yang sama baiknya terhadap skor indeks ulkus lambung dan kadar serum MDA. Akan tetapi akupunktur tanam benang memiliki efisiensi waktu dibandingkan dengan elektroakupunktur.
Kata kunci: Elektroakupunktur, Akupunktur tanam benang, tukak lambung, protektif


Introduction: Gastric ulcer is one of the most common diseases of the digestive tract which has a high recurrence rate. Treatment of gastric ulcers is often difficult and expensive. Pharmacological therapy has many side effects. Acupuncture as a nonpharmacological therapy has shown good results in therapy and as a protection against gastric ulcers. The purpose of this study was to compare the protective effect of electroacupuncture with thread implant acupuncture on gastric ulcer index and serum malondialdehyde (MDA) levels in gastric ulcers. Methods: The study was conducted in November - December 2021 at the Biomedical Research and Development Center, Ministry of Health of the Republic of Indonesia, Salemba, Central Jakarta. The research design is an experimental study with a randomized posttest design. 30 experimental rats were divided into 5 groups: normal group, gastric ulcer control (TL), omeprazole (OME), electroacupuncture (EA) and thread implant acupuncture (ATB). The OME group was given oral omeprazole 20 mg/kg and EA at ST36 and CV12 with a frequency of 2 Hz, the intervention for OME and EA was every 2 days for 12 days. The ATB group had 1 intervention on the first day. Gastric ulcer index scores and serum MDA levels were measured after gastric ulcer induction was performed 12 days after treatment. All data results were processed using SPSS version 20. Results: The ulcer index score was not significantly different between the EA and ATB groups (Mann Whitney test, p = 0.523), but the ulcer index scores of the EA and ATB groups were significantly lower than the TL group (Mann Whitney test, p < 0.05). Serum MDA levels were significantly lower in the EA versus TL group (post-hoc test, p < 0.001) and in the ATB versus TL group (post-hoc test, p < 0.05). In the EA versus ATB group, MDA levels were not significantly different (post-hoc test, p = 1,000). Conclusion: Electroacupuncture and thread implant acupuncture have a protective effect against gastric ulcers as well as gastric ulcer index scores and serum MDA levels. However, thread implant acupuncture has less time and cost efficiency than electroacupuncture.
Keywords: Electroacupuncture, thread implant acupuncture, gastric ulcer, protective