Tesis

Pengaruh Ekspresi miR-29a dan miR-31 terhadap Respons Radiasi pada Pasien Kanker Serviks Stadium Lanjut = Role of miR-29a and miR-31 expression on radiation response in advanced stage cervical cancer.

Latar Belakang: Respons terapi kanker serviks dipengaruhi oleh berbagai faktor seperti subtipe patologis, stadium klinis, dan status nodus limfe. Walaupun demikian, pasien dengan karakteristik yang sama dapat menunjukkan respons terapi yang berbeda. Hal ini dapat disebabkan oleh perbedaan faktor molekuler, salah satunya adalah miRNA yang berperan dalam regulasi ekspresi gen. miR-29a dan miR-31 mungkin berperan dalam terjadinya resistensi terhadap terapi kanker serviks. Objektif: Penelitian ini dilakukan untuk mengetahui kadar ekspresi miR-29a dan miR31 pasien kanker serviks terhadap respons radiasi. Metode: Penelitian ini merupakan studi prospektif. Sebanyak 20 sampel diambil dari pemeriksaan histopatologi jaringan serviks dan data rekam medis. Hasil biopsi jaringan serviks diperiksakan kadar ekspresi miR-29a dan miR-31. Analisis data menggunakan Mann Whitney dan Spearmen. Hasil: Penelitian menujukkan bahwa ekspresi miR-31 meningkat sebanyak 4,04 kali sedangkan ekspresi miR-29a terjadi penurunan sebesar 0.83 kali pada pasien yang tidak merespons terhadap radiasi. Peningkatan miR-31 tidak bermakna secara statistik (p=0,211), sedangkan penurunan miR-29a bermakna secara statistik (p=0,016, p < 0,05). Analisis lebih lanjut didapatkan bahwa usia, derajat differensiasi sel dan volume tumor serviks tidak menujukkan hasil signifikan terhadap kadar ekspresi miR-29a dan miR-31. Kesimpulan: Kadar ekspresi miR-29a dan miR-31 dapat digunakan sebagai salah satu prediksi respon radiasi terhadap pengobatan kanker serviks stadium lanjut walaupun secara statitistik tidak bermakna.
Kata kunci: Kanker Serviks Stadium lanjut, miR-29a, miR-31, respon radiasi


Backgound: The response to cervical cancer therapy is influenced by various factors such as pathological subtype, clinical stage, and lymph node. However, patients with the same characteristics may take response differently to therapy. This can be caused by differences in molecular factors, one of which is miRNA which plays a role in the regulation of gene expression. miR-29a and miR-31 may play a role in the development of resistance to cervical cancer therapy. Objectives: This study was conducted to determine the expression levels of miR-29a and miR-31 in cervical cancer patients to radiation response. Methods: This research is a prospective study. A total of 20 samples were taken from histopathological examination of cervical tissue and medical record data. The results of cervical tissue biopsy were examined for miR-29a and miR-31 expression levels. Data analysis using Mann Whitney and Spearmen. Results: The study showed that miR-31 expression increased by 4.04 times while miR29a expression decreased by 0.83 times in patients who did not respond to radiation. The increase in miR-31 was not significant statistically (p=0.211), while the decrease in miR-29a was significant statistically (p=0.016, p < 0.05). Further analysis found that age, degree of cell differentiation and cervical tumor volume did not show significant results on the expression levels of miR-29a and miR-31. Conclusions: The expression levels of miR-29a and miR-31 can be used as a predictor of radiation response to treatment of advanced cervical cancer although they are not significant statistically.
Keywords: Advanced cervical cancer, miR-29a, miR-31, radiation response