Skripsi

In Vivo Study of Curcumin Concentration in Colon of Rats Following Modification into Nano-sized Particles = Studi In Vivo Konsentrasi Kurkumin di Usus Besar Tikus Setelah Modifikasi Ukuran Kurkumin Menjadi Nanopartikel.

Introduction: Recent studies have demonstrated the ability of curcumin to inhibit carcinogenesis in colorectum. Despite much evidence of its medicinal benefits, curcumin has not yet been qualified as a therapeutic agent due to its poor absorption, bioavailability, stability, and rapid metabolism and elimination. Therefore. this research seeks to find out if modifying curcumin size into nanoparticles may improve its concentration in colon of rats. Method: This research is an experimental in vivo study in female Sprague-Dawley rats that were treated orally with 500mg/kg/BW of conventional curcumin and nanocurcumin. Samples of colon were obtained after 3 hours and 4 hours of administrations. Curcumin concentrations were measured using the UPLC-MS/MS method. Results: Concentration of conventional curcumin is slightly higher than nanocurcumin in the colon tissue after 3 hours and 4 hours of administration. No significant difference is seen statistically (p > 0.05). Mean curcumin and nanocurcumin concentration in rat colon after 3 hours was 92,463 ± 10.836 ug/g colon and 60.931± 4.773 ug/g colon respectively. Meanwhile, after 4 hours, mean curcumin dan nanocurcumin in rat colon was 113.560 ± 12.477 ug/g colon and 103.725 ± 12.951 ug/g colon respectively. Conclusion: Modification of curcumin size particles into nano-size does not improve its concentration in rat colon after oral administration. Possible explanations include aggregation of nanoparticles, anchoring of both types of particles in the mucus, penetration of nanoparticles across intestinal epithelium via transcellular transport, and higher chemical and biological degradation of nanocurcumin in the gastrointestinal tract. These result in a declined quantity of nanocurcumin that could be directly absorbed into the colon.
Keywords: colon, curcumin, nanoparticles, UPLC-MS/MS


Pendahuluan: Berbagai penelitian terdahulu telah membuktikan kemampuan kurkumin untuk menghambat karsinogenesis pada kolorektal. Namun pengembangan kurkumin untuk aplikasi ini terbatas dikarenakan penyerapannya dan bioavailabilitas yang buruk, sifatnya yang kurang stabil, serta tingkat metabolisme, dan eliminasi zat yang cepat. Oleh karena itu, penelitian ini dilakukan untuk membuktikan apakah modifikasi ukuran kurkumin menjadi nanopartikel dapat meningkatkan konsentrasi kurkumin di dalam kolon. Metode: Penelitian ini merupakan studi eksperimental pada tikus Sprague-Dawley betina yang diberikan kurkumin konvensional dan nano-kurkumin secara oral 500mg/kg/BB. Sampel kolon diambil 3 jam dan 4 jam setelah pemberian kurkumin. Konsentrasi kurkumin diukur dengan menggunakan UPLC-MS/MS. Hasil: Setelah 3 jam dan 4 jam pemberian sampel, ditemukan bahwa konsentrasi kurkumin konvensional cenderung lebih tinggi dibandingkan dengan konsentrasi nano-kurkumin, walau tidak ada perbedaan yang signifikan (p > 0.05). Rata-rata konsentrasi kurkumin dan nanocurcumin pada usus tikus setelah 3 jam adalah 92,463 ± 10.836 ug/g kolon dan 60.931± 4.774 ug/g kolon masing-masing. Sementara itu, setelah 4 jam, rata-rata konsentrasi curcumin dan nanocurcumin di usus tikus adalah 113.560 ± 12.477 ug/g kolon dan 103.725 ± 12.951 ug/g kolon masing-masing. Kesimpulan/Diskusi: Modifikasi ukuran kurkumin menjadi ukuran nano tidak mempengaruhi tingkat konsentrasi kurkumin dalam jaringan kolon tikus. Beberapa penyebab potensialnya adalah agregasi nano-partikel kurkumin, kedua jenis kurkumin terperangkap di dalam mukus, penetrasi nano-partikel melalui transportasi transeluler di dalam epitelium usus, dan tingginya degradasi nanokurkumin di dalam saluran pencernaan (baik secara biologis maupun kimiawi). Hal ini menyebabkan penurunan kuantitas nanokurkumin yang dapat diserap oleh kolon.
Keywords: kolon, kurkumin, nanopartikel, UPLC-MS/MS