Skripsi

Uji Aktivitas Antibiotik Senyawa Asam Galat dan Oktil Galat sebagai Penghambat Pertumbuhan Bakteri Salmonella thypi dan Streptococcus pneumoniae = Antibiotic Activity Tests for Galllic Acid and Octyl Gallate Compounds as Inhibitors of Salmonella thypi and Streptococcus pneumoniae Growth.

Asam galat dan oktil galat adalah senyawa yang telah banyak digunakan dalam berbagai industri. Asam galat diketahui memiliki potensi terapeutik yang menguntungkan yaitu sebagai antibakteri, antiviral, antiinflamasi, dan antikarsinogenik. Begitu pula dengan salah satu turunannya yaitu oktil galat. Turunan asam galat yang memiliki rantai karbon alkil sebanyak 8 ini berfungsi sebagai antibakteri, antifungal, dan antioksidan yang poten. Tingginya kasus resistensi terhadap bakteri Salmonella thypi dan Streptococcus pneumoniae memunculkan peluang bagi asam galat dan oktil galat sebagai alternatif antibiotik baru. Pengujian dilakukan dengan metode difusi cakram dilanjutkan dengan mengukur zona hambat yang terbentuk. Baik Salmonella thypi maupun Streptococcus pneumoniae, diujikan dengan asam galat dan oktil galat dengan konsentrasi 16 mg/L, 32 mg/L, 64 mg/L, 128 mg/L, 256 mg/L, 512 mg/L, dan 1024 mg/L. Tiap pengujian dilakukan pengulangan sebanyak tiga kali (triplo). Dalam pengujian ini digunakan kontrol positif yaitu disc gentamicin dengan konsentrasi 10 µg. Hasil penelitian dinyatakan dalam satuan mm berdasarkan zona hambat masing-masing senyawa. Dari hasil pengujian menggunakan 7 konsentrasi yang berbeda, asam galat dan oktil galat belum mampu menghambat aktivitas bakteri Salmonella thypi dan Streptococcus pneumoniae, karena teramati zona hambat 0 mm hingga konsentrasi 1024 mg/L.
Kata kunci : Salmonella thypi, Streptococcus pneumoniae, demam tifoid, pneumonia, asam galat, oktil galat


Gallic acid and octyl gallate are compounds that have been widely used in various industries. Gallic acid is known to have potential therapeutic benefits as an antibacterial, antiviral, antifungal, anti-inflammatory, and anticarcinogenic. Similar to one of its derivatives, octyl gallate. This gallic acid derivative which have 8 alkyl carbon chains, functions as antibacterial, antifugal, and potent antioxidant. The high cases of resistance of Salmonella thypi and Streptococcus pneumoniae give opportunities for gallic acid and octyl gallate as an alternative to new antibiotics. The test was carried out by disc diffusion method followed by measuring the inhibition zone formed. Both Salmonella thypi and Streptococcus pneumoniae were tested with gallic acid and octyl gallate with a concentration of 16 mg/L, 32 mg/L, 64 mg/L, 128 mg/L, 256 mg/L, 512 mg/L, and 1024 mg/L. Each test were repeated three times (triplo). In this test, gentamicin disc 10 µg was used as the positive control. The results of this study are expressed in mm units based on the inhibition zones of each compound. From the results of testing using 7 different concentrations, gallic acid and octyl gallate have not been able to inhibit the activity of both Salmonella thypi and Streptococcus pneumoniae, because the observed inhibition zone is 0 mm to a concentration of 1024 mg/L.
Key word: Salmonella thypi, Streptococcus pneumoniae, typhoid fever, pneumonia, gallic acid, octyl gallate