Skripsi

Kemampuan Antioksidan Ekstrak Daun Pandan Wangi (Pandanus amaryllifolius) terhadap hati Tikus Jantan yang diberi Karbon Tetraklorida (CCl4) = Antioxidant Capacity of Pandanus amaryllifolius Leaf Extract against CCl4-induced Liver injuries in Rats.

Peningkatan radikal bebas dapat mengakibatkan sejumlah besar masalah kesehatan penyakit degeneratif dan penyakit tidak menular (PTM) yang memiliki prevalensi tinggi di Indonesia dan merupakan masalah kesehatan di dunia. Daun Pandan Wangi (Pandanus amaryllifolius) merupakan salah satu tanaman yang banyak tumbuh di Asia khususnya Indonesia dan memiliki kandungan yang bersifat antioksidan yakni flavonoid, alkaloid, dan fenolik. Penelitian ini mengkaji kemampuan antioksidan ekstrak etanol daun pandan wangi pada hati tikus yang diberi CCl4. Kondisi stres oksidatif dipicu dengan pemberian CCL4 0,55 mg/kgBB, 24 tikus Sprague-Dawley jantan dibagi menjadi 4 kelompok kontrol, CCl4, ekstrak etanol daun pandan wangi, ekstrak etano l daun pandan wangi +CCl4. Dosis ekstrak yang digunakan yakni 85 mg/KgBB. Kemampuan antioksidan ekstrak pandan dinilai dari pengukuran penanda stres oksidatif (MDA, GSH, dan aktivitas spesifik katalase). Hasil penelitian ini menunjukkan tidak ada perbedaan yang signifikan antar kelompok (p > 0,05). Berdasarkan perbedaan reratanya, pemberian ekstrak daun pandan wangi menurunkan kadar MDA hati dan meningkatkan kadar GSH dan aktivitas spesifik katalase hati yang diinduksi oleh CCl4 secara tidak signifikan dibanding kelompok CCl4. Pemberian CCl4 0,55 mg/kgBB menunjukkan peningkatan kadar MDA hati dan penurunan aktivitas spesifik katalase dan GSH yang tidak signifikan dibanding kelompok kontrol. Dari hasil yang didapat masih dibutuhkan penelitian lebih lanjut dengan dosis CCl4 yang lebih tinggi dan membandingkan efek pemberian ekstrak pandan dengan kontrol positif vitamin E.
Kata kunci : Pandanus amaryllifolius, Antioksidan, Malondialdehida, Glutation, Katalase, Karbon tetraklorida


Increased production of free radicals can lead to a large number of health problems of degenerative and non-communicable diseases (NCD) which have a high prevalence in Indonesia and common health problem in the world. Pandan Leaf Extract (Pandanus amaryllifolius) is one of the plants that grows in Asia, especially in Indonesia. Major compounds that act as antioxidants found in pandan leaf is flavonoids, alkaloids, and phenolics. This study investigated the antioxidant capacity of ethanolic extract of pandan leaves on the CCl4-induced liver of rats. The condition of oxidative stress was triggered by the administration of 0,55 mg / kgBW CCL4, 24 male Sprague-Dawley rats were divided into 4 groups,which were given normal condition(no intervention), CCl4, ethanolic extract of pandan leaves, ethanolic extract of pandan leaves + CCl4. The dose of extract used is 85 mg/KgBW. The antioxidant capacity of pandan extract was assessed by measuring oxidative stres markers (MDA, GSH, and catalase specific activity). The results of this study showed no significant differences between groups (p > 0,05). Based on differences in mean, administration of pandan leaf extract decreased liver MDA levels and increased GSH levels and catalase specific activity of liver induced by CCl4 insignificantly compared to CCl4 group. The administration of 0,55 mg/kgBW CCl4 demonstrated an increase in liver MDA levels and a decrease in catalase specific activity and GSH level which was not significant compared to the control group. From the results obtained, further research is needed by using higher dose of CCl4 and also comparing the effect of administrating pandan extract with positive control (Vitamin E).
Keywords : Pandanus amaryllifolius, Antioxidants, Malondialdehyde, Glutathione, Catalase, Carbon Tetrachloride