Skripsi

Perbedaan ekspresi gen KLF4 di Adipose Derived Stem Cell (ADSC) dan Umbilical Cord Stem Cell (USC) = The difference in expression of gene KLF4 in Adipose Derived Stem Cell (ADSC) and Umbilical Cord Stem Cell (USC).

Background: Recently, stem cells have garnered popularity as regenerative therapy to degenerative diseases that are prevalent in the aging population. Based on pluripotency, Embryonic Stem Cells (ESC) are immensely useful for replacement therapies. However, given the ethical issues and immune barrier, the possibilities of using other type of stem cells such as Adipose Derived Stem Cells (ADSC) and Umbilical Cord Stem Cells (USC) are being explored. As the pluripotency of these two cells have yet to be studied thoroughly, this research aims to analyse the pluripotency level of the two type of stem cells through the expression of KLF4 gene, a co-regulator of pluripotency network. Method: RNA was extracted from both ADSC and USC. One-step real-time RTPCR was performed to find the relative expression of KLF4 gene which depicts the pluripotency level of each stem cell. Results: Relatively, KLF4 is expressed higher in ADSC as compared to USC. Conculsion: ADSC has higher pluripotency level than USC while USC should have higher pluripotency. This could be due to the roles of other genes that are involved in pluripotency core network.
Keywords: adipose derived stem cell, umbilical cord stem cell, KLF4, gene expression


Latar Belakang: Belakangan ini, terapi regeneratif menggunakan sel induk menjadi sebuah terapi populer terhadap penyakit degeneratif yang lazim terpapar pada populasi lanjut usia. Berdasarkan konsep pluripotency, Embryonic Stem Cells (ESC) sangat bermanfaat untuk terapi regeneratif ini. Namun, mengingat masalah etis dan autoimun, kemungkinan menggunakan jenis sel induk lainnya seperti Adipose Derived Stem Cells (ADSC) dan Umbilical Cord Stem Cells (USC) sedang dieksplorasi. Karena pluripotency dari kedua sel ini belum dipelajari secara menyeluruh, penelitian ini bertujuan untuk menganalisis tingkat pluripotency dari dua jenis sel induk melalui ekspresi gen KLF4, sebuah gen yang merupakan co-regulator jaringan pluripotency. Metode: RNA diekstraksi dari ADSC dan USC. One-step real-time RT-PCR dilakukan untuk mendapatkan ekspresi relatif gen KLF4 yang menggambarkan tingkat pluripotency sel induk tersebut. Hasil: Secara relatif, ekspresi KLF4 dinyatakan lebih tinggi di ADSC dibandingkan dengan USC. Konklusi: ADSC memiliki tingkat pluripotency yang lebih tinggi daripada USC sementara USC seharusnya memiliki pluripotency yang lebih tinggi. Ini bisa jadi karena peran gen lain yang terlibat dalam jaringan inti pluripotency
Kata kunci: adipose derived stem cell, umbilical cord stem cell, KLF4, gene expression