Disertasi

Pengaruh yogurt yang diperkaya dengan probiotik La5 Bb12 : dan prebiotic jali terhadap kadar GLP-1, Glycated albumin, kadar butirat dan calprotectine penyandang DM tipe 2.

International Diabetes Federation (IDF) pada tahun 2014 menyatakan penyandang DM yang berusia 20–79 tahun sejumlah 8% dari seluruh penduduk Indonesia. Jali merupakan tumbuhan serealia yang mengandung 24,9 gram fruktooligosakarida dari setiap 100 gram bijinya.. Perpaduan satu porsi yogurt jali yang terdiri dari 100 ml yogurt, mengandung probiotik Lactobacillus acidophilus dan Bifidobacterium masing masing sebesar 109cfu/mL, dicampur dengan 25 g jali rebus dapat menurunkan inflamasi epitel usus yang ditandai dengan penurunan kadar Calprotectine (CP) feses, meningkatkan kadar Glucagon Like Peptide-1 (GLP-1) dan menurunkan resistensi insulin yang berperan dalam mengontrol kadar glukosa darah. Penelitian ini menggunakan randomized controlled trial parallel group study, untuk menilai pengaruh pemberian yogurt dan yogurt jali selama 12 minggu pada 60 subjek penyandang DM tipe2 masing- masing sebanyak 30 subjek pada kelompok yogurt dan yogurt jali. Didapatkan penurunan rerata BB (p < 0,001) pada kelompok yogurt jali dan yogurt dan terdapat perbedaan (p=0,02) delta berat badan (BB) subjek pasca intervensi antar kedua kelompok. Kadar GLP-1 subjek penelitian kelompok yogurt maupun yogurt jali tidak mengalami peningkatan. Terdapat perbedaan (p < 0,001) di antara delta kadar Glukosa Darah Puasa (GDP) kedua kelompok tersebut. Kadar CP feses pra dan pasca intervensi pada subjek kelompok yogurt jali terdapat perbedaan yang bermakna (p < 0,05), namun tidak berbeda pada subjek kelompok yogurt. Pemberian yogurt jali dapat menurunkan berat badan, GDP, GLP-1 dan CP dibandingkan pemberian yogurt.
Kata kunci : DM tipe 2, yogurt jali, Glycated albumin, calprotectine, GLP-1.


Background: International Diabetes Federation (IDF) in 2014 reported that diabetes mellitus prevalence in Indonesia were 8%.Job’s tears is a cereal containing 24.9 grams of fructooligosaccharide per 100 grams of dry seed. The combination of one serving of yogurt with Job’s tears composed from 100 ml yogurt, containing Lactobacillus acidophilus and Bifidobacterium probiotics of 109cfu / mL each, mixed with 25 g of boiled Job’s tears could decrease the inflammation of the intestinal epithelium, decrease the oxidative stress and insulin resistance that will play a role in controlling blood glucose. Method: This study used randomized controlled trial parallel group study to evaluate the effect of yogurt and yogurt with Job’s tears on FBG, GLP-1, CP and body weight in type 2 diabetes mellitus. The subjects in this study were 60 employees of Atma Jaya Jakarta with randomization block, each of 30 subjects in intervention (yogurt with Job’s tears) and control (yogurt only) groups. Result: A reduced body weight (p < 0,001) in both groups of yoghurt with Job’s tears and control was observed. There was a significant difference (p = 0,02) of delta body weight between both groups of the subjects after intervention We could not find a significant increase of the level of GLP-1 in both groups There was a significantly differen (p < 0.001) of the delta FBG levels of both groups. CP levels in the pre- and post-intervention’s stools on the intervention groups were significantly different (p < 0.05), but notin the control group. Conclusion: Yogurt with Job’s tears could reduce body weight, FBG, GLP-1 and CP compared to common yogurt on type 2 diabetes mellitus patients.
Keyword: yogurt, Job’s tears, diabetes mellitus, weight, FBG, GLP-1, CP.