Tesis

Peran Sel T Regulator pada Pasien HIV dengan TB Aktif dan Laten = The Role of Regulatory T Cells in HIV Patients with Active and Latent Tuberculosis.

elah dilakukan suatu penelitian eksperimental untuk menilai peran sel T regulator pada pasien dengan ko-infeksi HIV-TB. Terhadap 18 subyek HIV positif dilakukan penilaian IGRA, isolasi dan kultur PBMC dengan stimulasi antigen MTB, serta sorting dan deplesi Treg CD4+CD25 high CD127 low dengan metode FACS. Produksi sitokin IFN-γ dan IL-10 dinilai secara kuantitatif dengan multiplex Luminex 200. Diperoleh sebanyak 10 (55,6%)subyek TB negatif, 6 (33,3%) subyek TB laten, dan 2 (11,1%) subyekTB aktif. Persentase Treg dari CD4+ pada subyek HIV dengan status TB menunjukkan kenaikan signifikan dibanding nilai referensi batas atas persentase Treg dalam CD4 subyek normal (11,006 % ± 2,840 %; p=0,008). Rerata persentase Treg dari sel PBMC total antara kelompok TB aktif dan TB negatif menunjukkan perbedaan yang signifikan (1,3% vs 0,8%; p=0,036). Tidak terdapat perbedaan rasio sitokin proinflamasi (INF-γ) terhadap sitokin anti inflamasi (IL-10) pada kelompok dengan ko infeksi HIV- TB aktif dan laten sebelum dan sesudah deplesi Treg. Kata Kunci: Treg, HIV, TB, Ko-infeksi, IL-10, IFN-γ


An experimental study has been conducted to assess the role of T regulator cells in patients with HIV-TB co-infection. 18 HIV positive subjects undergo IGRA assessment, PBMC isolation and culture with ESAT-6/CFP-10 micobacterial antigen stimulation, and Treg CD4 + CD25 high CD127 low sorting and depletion by FACS method. The production of cytokines IFN-γ and IL-10 were quantitatively assessed with Luminex 200 multiplex assay. Respectively, 10 (55.6%) were negative TB subjects, 6 (33.3%) were latent TB subjects, and 2 (11.1%) subjects were TB active. The percentage of Treg from CD4+ cells in HIV subjects with TB status showed a significant increase over the reference value in normal subjects (11.006% ± 2.840%; p = 0.008). The mean percentage of Treg from total PBMC cells between active and negative TB groups showed a significant difference (1.3% vs. 0.8%; p = 0.036). There was no difference in the ratio of proinflammatory cytokines (INF-γ) to the anti-inflammatory cytokine (IL-10) in the group with active and latent HIV-TB infection coinfection before and after Treg depletion.
Key words: Treg, HIV, TB, Co-infection, IL-10, IFN-γ