Skripsi

Potensi fraksi etanol Calophyllum nodusum sebagai antivirus dengue secara in vitro = The potency of ethanol fraction of Calophyllum nodusum as antiviral to dengue virus in vitro.

Demam berdarah dengue (DBD) merupakan masalah kesehatan di dunia termasuk di Indonesia. DBD disebabkan oleh dengue virus (DENV) yang ditransmisikan melalui nyamuk. Manifestasi klinis infeksi DENV dapat berupa demam dengue, DBD dan dapat berlanjut menjadi dengue shock syndrome yang akan menyebabkan kematian. Hingga saat ini usaha preventif berupa vaksin dengue. Tata laksana kuratif hanya bersifat suportif. Calophyllum nodusum merupakan tanaman yang tumbuh di Indonesia memiliki efek sebagai anti mikroba. Potensi C. Nodusum sebagai antivirus DENV pelu diteliti. Penelitian antivirus DENV dilakukan dengan metode in-vitro menggunakan fraksi etanol ekstrak C. Nodusum, DENV-2 strain New Guinea C (NGC) dan sel Huh7it-1. Pengujian efek antivirus dengan menggunakan metode focus assay sedangkan uji sitotoksisitas terhadap sel menggunakan 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Nilai half inhibitory concentration (IC 50 ekstrak C. Nodusum sebesar 2,12 µg/ml serta nilai half cytotoxic concentration (CC 50 sebesar 54,01 µg/ml. Nilai indeks selektivitas adalah 25,48. Pada hasil focus assay tidak terlihat adanya perbedaan bermakna antara kelompok perlakuan dengan kontrol DMSO 0,1% (p>0,01). C. Nodusum memiliki potensi sebagai kandidat antivirus DENV.
Kata kunci: demam dengue, demam berdarah dengue, dengue shock syndrome, vaksin dengue, Calophyllum nodusum, dengue virus, DENV-2, sel Huh7it-1


Dengue hemorrhagic fever (DHF) is one of the world’s health problem, as well in Indonesia. DHF caused by dengue virus (DENV) that transmitted by mosquito. Clinical manifestations of DENV infection is dengue fever, DBD and dengue shock syndrome that leads to death. Until now, the preventive method is using dengue vaccine. The curative method is still using supportive ways. Calophyllum nodusum is a plants that growth in Indonesia and also has anti-microbial effect. The potency of C. nodusum as antiviral of DENV needs to be examined. This antiviral of DENV research use ethanol fraction from C. nodusum, DENV-2 New Guinea C (NGC) strain, Huh7it-1 cell with in-vitro method. The antiviral effect examined by using focus assay method while cytotoxicity effect examined by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay method. The value of half inhibitory concentration (IC 50 ) from C. nodusum extract is 2,12 µg/ml and the value of half cytotoxic concentration (CC 50 is 54,01 µg/ml. the value of selectivity index is 25,48. In focus assay method shows that there is no significance difference between tested variable and DMSO 0,1% as control (p>0,01). C. nodusum has potency as the candidate of DENV antiviral.
Key words: dengue fever, dengue hemorrhagic fever, dengue shock syndrome, dengue vaccine, Calophyllum nodusum, dengue virus, DENV-2, Huh7it-1 cel