Skripsi

In Vitro Study of Cassia alata Leaf Extract on the Replication of Dengue Virus Serotype 2 = Studi In Vitro Ekstrak Daun Cassia alata pada Virus Dengue Serotipe-2.

Background : Dengue fever has been causing health related issues and distress all around the world, especially in developing countries such as Indonesia. Up to date, there has yet to be an effective antiviral to eliminate Dengue Virus Serotype-2 (DENV-2. Cassia alata leaves extract are known for its rich compounds that are thought to be beneficial as an antiviral. Methods : The Cassia alata extracts were tested upon Huh7 cells that are infected with DENV-2 and various concentrations of the extract (320 µg/mL, 160 µg/mL, 80 µg/mL, 40 µg/mL, 20 µg/mL, and 10 µg/mL) were given to treat the cells, with DMSO 0.1% as a negative control. Cell viability was assessed using MTT Assay and the viral inhibition was evaluated using Focus Assay, through immunostaining procedure. Result : The extract on Huh-7 cells had a CC50 of