Skripsi

Perbandingan Sensitivitas dan Spesifisitas Uji Diagnostik Pewarnaan Basil Tahan Asam terhadap BACTEC Mycobacteria Growth Indicator Tube 960 = Comparison of Sensitivity and Specificity in Diagnostic Test of Acid Fast Bacilli Smear to BACTEC Mycobacteria Growth Indicator Tube 960.

Mayoritas kasus tuberkulosis yang tidak terdiagnosis memiliki hasil sputum basil tahan asam negatif. Pembiakan Mycobacteria Growth Indicator Tube diperhitungkan sebagai penunjang pemeriksaan basil tahan asam dalam konfirmasi diagnosis tuberkulosis. Penelitian ini bertujuan untuk mengetahui sensitivitas dan spesifisitas pewarnaan basil tahan asam terhadap BACTEC Mycobacteria Growth Indicator Tube 960. Penelitian ini menggunakan studi uji diagnosis pada 188 sampel yang memenuhi kriteria penelitian dari Laboratorium Departemen Mikrobiologi Klinik Fakultas Kedokteran Universitas IndonesiaRumah Sakit Cipto Mangunkusumo (FKUI-RSCM). Data dianalisis secara komparatif kategorik berpasangan dengan uji McNemar. Dari 188 sampel (107 laki-laki dan 31 perempuan dengan median usia 35[17-76 tahun), hasil positif basil tahan asam (scanty, 1+, 2+, 3+) dan BACTEC Mycobacteria Growth Indicator Tube 960 berturut-turut adalah (32,9%) 62 sampel dan (59,6%) 112 sampel. Sensitivitas pewarnaan basil tahan asam terhadap BACTEC Mycobacteria Growth Indicator Tube 960 berbeda signifikan (p=0,000 [8,1371e-13]). Melalui tabel 2x2 diketahui sensitivitas, spesifisitas, nilai duga positif, dan nilai duga negatif pewarnaan basil tahan asam terhadap BACTEC Mycobacteria Growth Indicator Tube 960 berturut-turut adalah 52,7%; 96,1%; 95,1%; dan 57,9%. Pemeriksaan BACTEC Mycobacteria Growth Indicator Tube 960 memiliki sensitivitas dan spesifisitas yang lebih unggul daripada pemeriksaan BTA dan dapat digunakan untuk menunjang pemeriksaan BTA sebagai uji diagnostik TB.
Kata kunci: Sensitivitas, spesifisitas, uji diagnostik, MGIT, BTA, tuberkulosis paru


The majority of undiagnosed tuberculosis cases have negative acid-fast bacilli result. Culture with Mycobacteria Growth Indicator Tube is considered as a support to acid-fast bacilli smear in confirmation of tuberculosis diagnosis. This study aims to determine the sensitivity and spesificity of acid-fast bacilli smear against BACTEC Mycobacteria Growth Indicator Tube 960. This study used a diagnostic test study in 188 samples that met the study criteria obtained from Clinical Microbiology Laboratory of Faculty of Medicine Universitas IndonesiaCipto Mangunkusumo Hospital (FMUI-RSCM). Data were analyzed with McNemar test. Out of 188 samples (107 males and 31 females with an average age of 35[17-76] years), positive results for acid-fast bacilli (scanty, 1+, 2+, 3+) and BACTEC Mycobacteria Growth Indicator Tube 960 respectively were (32.9%) 62 samples and (59.6%) 112 samples. The sensitivity of acid-fast bacilli smear against BACTEC Mycobacteria Growth Indicator Tube 960 is significantly different (p=0,000 [8,1371e-13]). Through the 2x2 table, the sensitivity, specificity, positive and negative predictive value respectively were 52.7%, 96.1%, 95.1%, and 57.9%. BACTEC Mycobacteria Growth Indicator Tube 960 has superior sensitivity than acid-fast bacilli smear and can be used to support acidfast bacilli smear in diagnosing tuberculosis cases.
Keywords: Sensitivity, specificity, diagnostic test, MGIT, BTA, lung tuberculosis