Tesis

Korelasi antara Ekspresi Podoplanin pada Cancer Associated Fibroblast (CAF) dan Lymphatic Microvessel Density (LMVD) dengan Metastasis Kelenjar Getah Bening pada Adenokarsinoma dan Adenokarsinoma Not Otherwise Specified (NOS) Kolorektal = Correlation between Podoplanin Expression in Cancer Associated Fibroblast (CAF) and Lymphatic Microvessel Density (LMVD) with Lymph Node Metastasis in Mucinous Adenocarcinoma and Not Otherwise Specified (NOS) Colorectal Adenocarcinoma.

Latar Belakang: Adenokarsinoma musinosum mempunyai prognosis lebih buruk dari pada adenokarsinoma Not Otherwise Specified (NOS) kolorektal. Ekspresi podoplanin pada Cancer associated fibroblasts (CAF) dan nilai Lymphatic Microvessel Density (LMVD) yang tinggi merupakan petanda prognosis yang buruk. Pada penelitian ini dilakukan analisis terhadap CAF, LMVD dan metastasis kelenjar getah bening (KGB) pada adenokarsinoma musinosum dan adenokarsinoma NOS. CAF merupakan komponen utama pada stroma desmoplastik yang memiliki peran penting dalam proses metastasis. LMVD merupakan penilaian densitas pembuluh limfatik pada massa tumor primer. Bahan dan Metode: Penelitian dilakukan terhadap 44 kasus yang terdiri atas 22 kasus adenokarsinoma musinosum dan 22 kasus adenokarsinoma NOS kolorektal. Pulasan podoplanin digunakan untuk melihat ekspresinya pada CAF dan nilai LMVD. Hasil: CAF yang terekspresi podoplanin pada adenokarsinoma NOS lebih tinggi daripada adenokarsinoma musinosum kolorektal (p=0,000). Tidak terdapat perbedaan bermakna nilai LMVD antara adenokarsinoma NOS dengan adenokarsinoma musinosum kolorektal (p=0,381). Tidak terdapat korelasi antara CAF yang terekspresi podoplanin dengan nilai LMVD pada adenokarsinoma musinosum (p=0,248) dan adenokarsinoma NOS (p=0,448). Tidak terdapat korelasi antara CAF yang terekspresi podoplanin dengan metastasis KGB pada adenokarsinoma musinosum (p=0,240) dan adenokarsinoma NOS (p=0,791). Kesimpulan: Ekspresi podoplanin pada CAF pada adenokarsinoma NOS lebih tinggi daripada adenokarsinoma musinosum. Tidak terdapat korelasi antara CAF yang terekspresi podoplanin dengan LMVD dan metastasis KGB pada adenokarsinoma musinosum dan adenokarsinoma NOS. Ekspresi podoplanin pada CAF tidak dapat digunakan untuk menentukan prognosis metastasis KGB pada adenokarsinoma musinosum. Terdapat kecenderungan nilai LMVD pada adenokarsinoma musinosum sedikit lebih tinggi daripada adenokarsinoma NOS kolorektal.
Kata Kunci: Adenokarsinoma musinosum, Adenokarsinoma Not Otherwise Specified (NOS), Cancer Associated Fibroblast (CAF), Lymphatic Microvessel Density (LMVD), Podoplanin


Background: Mucinous adenocarcinoma has worse prognosis than the Not Otherwise Specified (NOS) colorectal adenocarcinoma. High expression of podoplanin in Cancer Associated Fibroblasts (CAF) and high lymphatic Microvessel Density (LMVD) values is a marker of poor prognosis. This study analyzed CAF, LMVD and lymph node metastasis in mucinous adenocarcinoma and NOS adenocarcinoma.CAF are the main component of desmoplastic stroma which have an important role in the metastatic processes. LMVD is an assessment of the density of the lymphatic vessels in the primary tumor. Materials and methods: The study consists of 44 cases including 22 cases of mucinous adenocarcinoma and 22 cases of NOS adenocarcinoma colorectal. Podoplanin is used to see its expression CAF and LMVD values. Results: CAF expressed podoplanin at NOS adenocarcinoma is higher than mucinous adenocarcinoma (p=0.000). There was no significant difference in LMVD values between NOS adenocarcinoma and mucinous adenocarcinoma (p=0.381). There was no correlation between CAF expressed podoplanin with LMVD values in mucinous adenocarcinoma (p=0.248) and NOS adenocarcinoma (p=0.448). There was no significant correlation between CAF expressed podoplanin with KGB metastasis in adenocarcinoma musinosum (p=0,240) and NOS adenocarcinoma (p=0.791). Conclusion: CAF expressed podoplanin at NOS adenocarcinoma is higher than that of the mucinous adenocarcinoma. There is no correlation between CAF expressed podoplanin with LMVD and KGB metastasis in NOS mucinous adenocarcinoma and NOS adenocarcinoma. Podoplanin expression in CAF can not be used to determine the prognosis of KGB metastasis in mucinous adenocarcinoma. There is a tendency for LMVD values in mucinus adenocarcinoma to be slightly higher than NOS adenocarcinoma.
Key words: Mucinous adenocarcinoma, Not Otherwise Specified (NOS) Adenokarsinoma, Cancer Associated Fibroblast (CAF), Lymphatic Microvessel Density (LMVD), Podoplanin