Tesis

Analisis Proliferasi dan Diferesiansiasi Sel Punca Wharton's Jelly Tali Pusat Manusia dari Persalinan Preterm = Analysis of proliferation and differentiation of Wharton's jelly derived stem cells from preterm human umbilical cords.

Latar Belakang : Sel punca mesenkimal (SPM) sangat menjanjikan untuk pengobatan penyakit degeneratif. Keterbatasan penggunaan sel punca dari jaringan embrionik dan dewasa menyebabkan peneliti mencari alternatif lain sumber sel punca, salah satunya wharton’s jelly tali pusat. Wharton’s jelly (WJ) dari persalinan aterm (cukup bulan) telah berhasil diisolasi dan didiferensiasikan, sedangkan WJ dari persalinan preterm (kurang bulan) belum banyak dilakukan. Penelitian ini bertujuan untuk melihat kemampuan WJ sebagai sumber sel punca dan membandingkan proliferasi dan diferensiasi WJ dari persalinan preterm dan aterm menggunakan medium kultur xenofree. Metode: Sel punca WJ dikultur dalam medium DMEM 10% FBS, DMEM 10% PRP dan Mesencult®. Sel yang telah konfluens dipanen, dan ditumbuhkan kembali pada wadah yang baru (pasase) dengan medium yang sama. Pasase dilakukan hingga pasase ke 5 dan dilakukan uji diferensiasi pada pasase 3 dan 5. Jumlah sel antara WJ dari persalinan preterm dan aterm dianalisis menggunakan analisis statistik t-independent test. Hasil : WJ preterm tumbuh dan bersifat plastic-adherent dan memiliki perbedaan yang tidak bermakna dalam proliferasi sel dengan jumlah populasi sel lebih besar dibandingkan WJ aterm. Sel punca WJ preterm dapat berdiferensiasi dan medium xenofree dapat digunakan untuk menggantikan FBS Kesimpulan: WJ dari persalinan pretem dapat digunakan sebagai sumber sel punca mesenkimal.
Kata Kunci: sel punca, wharton’s jelly, persalinan preterm, proliferasi, diferensiasi.


Background: Mesenchymal stem cells are claimed as a promising degenerative medicine. Due to limited use of stem cells derived from embryonic and adult tissues, researchers have to find alternative sources of stem cells, and one of them are Wharton's Jelly umbilical cord. Wharton's Jelly (WJ) derived from full-term birth have been isolated and differentiated, but very few researches focus on the WJ derived from preterm birth. This study aimed to analyse the ability of WJ a source for the stem cells, and to compare the proliferation and differentiation of WJ derived stem cells from preterm and full-term birth using xeno-free culture media. Method: WJ was cultured with the followings media: DMEM 10% FBS, DMEM 10% PRP and Mesencult®. Cells reaching confluence were harvested and pasage in different containers, but with the same media. Cell passaging was carried out until the fifth passage, and the differentiation tests were performed on the third and fifth passages. Cell cumulative between WJ derived stem cells from preterm and full-term birth were then analysed using t-independent test. Results: The preterm WJ grown in culture media were plastic-adherent, had a non-significant difference with WJ derived from full-term birth, but had a higher number of cell populations than the latter. WJ were able to differentiate, and xenofree media can be used to replace FBS. Conclution: WJ derived stem cells from preterm birth can be used as a source for mesenchymal stem cells. Key Words: stem cells, Wharton’s jelly, preterm birth, proliferation, differentiation.