Tesis

Kadar Benzo(a)pyrene-Protein Adducts pada Perokok Kretek dan Bukan Perokok serta Faktor yang Mempengaruhi = Benzo(a)pyrene Diol Epoxide(BPDE) Serum Level in Kretek Cigarette Smokers and Nonsmokers and the Influence Factors.

Latar Belakang : Benzopyrene (polycyclic aromatic) adalah agen karsinogenik yang ditemukan dalam asap rokok. Benzo(a)pyrene Diol Epoksid (BPDE) adalah salah satu metabolit benzopyrene. Perokok kretek adalah orang yang merokok minimal 1 rokok kretek per hari selama minimal 1 tahun tanpa atau kurang dari 20% nya merokok dengan rokok putih. Kami meneliti kadar BPDE dalam darah perokok kretek dibandingkan dengan bukan perokok. Metode : Penelitian ini menggunakan desain potong lintang yang mengikutsertakan 32 subjek sehat yang merokok kretek dan 32 subjek sehat bukan perokok. telah menandatangani lembar persetujuan diperiksakan kadar CO ekshalasi dengan menggunakan alat pengukur CO portabel kemudian diambil sampel darah untuk diukur kadar BPDEprotein adducts. Data yang diperoleh diuji dengan menggunakan statistical package for social sciences (SPSS) 21. Hasil : Total 64 subjek terbagi 32 subjek perokok kretek dan 32 kontrol diperiksakan kadar BPDE-protein adducts dan kadar CO ekshalasi. Kadar BPDE- protein adducts pada subjek perokok kretek sebesar 12,15(8,8733,55) ng/ml dan kadar pada kelompok kontrol sebesar 11,4(3,87-13,27) ng/ml. p=0,004. Faktor yang paling mempengaruhi kadar BDPE-protein adducts pada perokok kretek berdasarkan analisis multivariat adalah pola hisapan (p=0,002) dan derajat adiksi (p = 0,047). Terdapat hubungan yang bermakna secara statistis antara kadar BPDE-protein adducts dengan kadar CO ekshalasi pada perokok kretek (p=0,003). Kesimpulan : Kadar BPDE-Protein adducts pada perokok lebih tinggi dibanding bukan perokok dengan pola hisapan dan derajat adiksi menjadi faktor yang mempengaruhi
Kata Kunci : Benzo(a)pyrene, Perokok, CO ekshalasi, BPDE-protein adducts.


Background: Benzopyrene (polycyclic aromatic)is a carcinogenic agent found in cigarettes smoke. Benzo(a)pyrene Diol Epoxide (BPDE) is one of the benzopyrene metabolite. Kretek cigarette smoker isa person who smokes at least 1 cigarette per day for at least 1 year with no or less than 20% of amount and time of white cigarettes smoking. We investigated the BPDE serum level in kretek cigarette smokers compared to non-smokers Method : This is a cross sectional study with 32 “healthy”kretek cigarette smokers and 32 “healthy” non-smoker were enrolled in this study. We collected blood sample and we analyzed the BPDE level and also their exhaled carbon- monoxide (CO) level during expiration. Serum BPDE level were assayed using ELISA kit. The data obtained were tested using statistical package for social sciences (SPSS) 21. Results: From 64 respondent who divided into 32 kretek cigarette smokers subjects and 32 nonsmokers were underwent examination of BPDE-protein adducts level and exhaled CO levels. The levels of BPDEprotein adducts in the kretek cigarette smoker subjects were 12,15 (8,87 to 33,55) ng / ml and the levels in the nonsmokers group were 11,4 (3,87 to 13,27) ng / ml, p = 0.004. The factors which influence the levels of BDPEprotein adducts in kretek cigarette smokers the most using multivariate analysis were sucking pattern (p = 0.002) and the degree of addiction (p = 0,047). There was a statistically significant relationship between the level of BPDE-protein adducts with exhaled CO levels in kretek cigarette smokers (p = 0,003). Conclusion : Serum BPDE-protein adducts level higher in smokers compared with nonsmokers with suction pattern and degree of addiction which are influence factors
Keywords: Benzopyrene, Smokers, CO exhalation, BPDE-protein adducts