Tesis

Hubungan uji tuberkulin dan polymerase chain reaction dengan BTA mikroskopis dan biakan mycobacterium tuberculosis dalam diagnosis tuberkulosis paru

Objective: In an attempt diagnosis pulmonary tuberculosis still increased continuously. Now additional examination pulmonary tuberculosis have been lack sufficient sensitivity and spesitivities. The aim of this study was to determine the validity of tuberculin skin testing (TST) and PCR toward assessment diagnosis pulmonary of tuberculosis with correlation between tuberculin skin testing to PCR with AFB microscopic and solid media culture of M. tuberculosis for the diagnosis of pulmonary tuberculosis. Method: A cross-sectional study, diagnostic test and analysis with Chi-Square test. Inclution criteria patient with pulmonary symptom include chronic cough 3 week with or without hemoptysis, chest pain, breathlessness and past history of ATA less than 1 month with nontuberculosis patient ( control). The general samples was examination Ro thorax, tuberculin skin testing, PCR, AFB microscopic and conventional culture. The golden standart is conventional culture test using Kudoh methode. Analysis this data with SPSS version 1 1 .0. Result: The study material comprised 121 samples from 127 sample. This sample include 61 sample from patient with probably active pulmonary tuberculosis and 60 control comprising healthy individuals. The sensitivity and spesitivity of tuberculin skin testing with cut-off point 15.8 mm greater was 33 % and 93 % on conventional culture test using Kudoh methode. PCR sensitivity was 100 % and spesitivity was 78%. Showed the positivity correlation between pulmonary tuberculosis and conventional culture as well as PCR and AFB microscopic, the conventional culture test. Conclusion: The sensitivity tuberculin skin testing less than PCR, AFB microscopic and conventional culture test. So that not enough to assessment diagnosis pulmonary tuberculosis. The sensitivity and spesitivity PCR was 100 % and 78 %. With the use of PCR test, we were able to detect diagnosis pulmonary tuberculosis more rapidly in less than 1 day, compared to average 8 week required for detection by conventional culture.
Key word: TST, AFB microscopic, conventional culture M. Tuberculosis, PCR