Skripsi

The effect of azoxymethane and dextran sodium sulfate induction to dysplasia of Balb/c and C3H strain mice colon tissue = Efek Induksi Azoxymethane dan Dextran Sodium Sulfate Terhadap Displasia Pada Jaringan Kolon Mencit.

Background:According to American Cancer Society in 2015, colorectal cancer is surpringsingly the third most common cause of death in western countries for both men and women. In 2015, it is estimated that there will be approximately 132.700 new cases of colorectal cancer only in United States. In recent years, many researchers show that colorectal carcinoma is one of the serious complications of inflammatory bowel disease (IBD). Thus, it brings potential to gain more knowledge regarding the possibility of chronic inflammation leading to colorectal cancer. The main objective in this research is that we want to get more understanding about the role of inflammation towards dysplastic changes based on duration of development of disease. Metods:We conduct this research using C3H and Balb mice which have been proven to represent the colon carcinogenesis process in human body. We divided mice into several cage based on date of sacrifice which were 2, 4 and 6 months. Each group got once a week injection of azoxymethane for one month and was followed by administration of dextran sulfate sodium during the last week of the first month. Then mice were sacrificed based on certain duration and we observed the colon macroscopically and microscopically using HE staining with high magnification. Results: Based on macrocopic observation, we can easily find nodules in 6 months groups while for 2 months groups, the nodules have not yet developed. From morphological changes, the severity of dysplasia is in accordance with the longevity of the disease. Based on Annova Test, we find out that the result shows p=0,009 (p