Skripsi

Aktivitas In Vitro Derivat Asam Galat Sebagai Inhibitor Pertumbuhan Sel Kanker Serviks HeLa = In Vitro Activity of Gallic Acid Derivatives as Inhibitor of Cervical Cancer HeLa Cells Growth.

Kanker serviks merupakan kanker dengan prevalensi tertinggi di Indonesia. Tatalaksana kanker serviks saat ini meliputi kemoterapi, radioterapi, dan pembedahan yang relatif mahal dan menimbulkan efek samping. Asam galat adalah senyawa alami yang berpotensi menjadi pengobatan alternatif kanker serviks. Penelitian ini bertujuan untuk mengetahui aktivitas penghambatan asam galat dan derivatnya pada pertumbuhan kultur sel HeLa, yang dinyatakan dengan nilai IC . Asam galat dan derivatnya dilarutkan dalam phosphate buffered saline (PBS), dibuat triplo dengan 8 variasi konsentrasi dalam rentang 0,067-8,533 µg/mL. Kultur sel HeLa diencerkan dan ditambahkan 10 mL PBS serta 1 mL dulbecco's modified eagle medium. Viabilitas sel diuji dengan MTT assay. Hasil yang diperoleh menunjukkan terdapat 9 senyawa derivat yang lebih poten sebagai antikanker serviks HeLa dibandingkan asam galat (IC 50 : 3,606 µg/mL), yaitu: propil galat (IC 50 : 0,0000674 µg/mL), trans-2-heksenil galat (IC 50 : 0,09583 µg/mL), cis-2-heksenil galat (IC 50 : 0,19 µg/mL), 2-hidroksi-benzil galat(IC 50 : 0,40 µg/mL), amil galat (IC 50 : 0,62 µg/mL), sekunder-amil galat (IC 50: 0,73 µg/mL),asam 4-cis-2-heksenil-oksi galat (IC 50: 1,31 µg/mL), asam-4-trans-2-heksenil-oksi galat (IC 50: 1,69 µg/mL), dan benzil galat (IC 50 : 2,98 µg/mL). Secara umum, hasil penelitian menunjukkan bahwa penambahan rantai alkil ester pada asam galat dapat meningkatkan sitotoksisitasnya terhadap sel HeLa.
Kata kunci : asam galat, alkil ester galat, alkil eter galat, sel HeLa


Cervical cancer is a cancer with the highest prevalence in Indonesia. Currently, the treatments of cervical cancer (chemotherapy, radiotherapy, and surgery) still have side effects and limitations. Gallic acid is a natural compound that could be developed as alternative treatment of cervical cancer. This study aims to determine anticancer activity of gallic acid and its derivatives on the growth of HeLa cell cultures measured by IC value. Gallic acid and its derivatives dissolved in phosphate buffered saline (PBS), and made into 8 concentrations (0.067 to 8.533 µg/mL). HeLa cells culture is diluted and added by 10 mL PBS and 1 mL dulbecco's modified eagle medium. HeLa cells viability was tested by MTT assay. Results showed 9 gallic acid derivatives that have greater anticancer activity than gallic acid (IC 50 : 3,606 µg/mL), i.e.: propyl gallate (IC 50 : 0,0000674 µg/mL), cis-2-hexenyl gallate (IC 50 : 0,19 µg/mL), 2-hydroxy-benzyl gallate (IC 50 : 0,40 µg/mL), amyl gallate (IC 50: 0,62 µg/mL), secamyl gallate (IC 50 : 0,73 µg/mL), 4-cis-2-hexenyl-oxy gallic acid (IC 50 : 1,31 µg/mL), 4-trans2-hexenyl-oxy gallic acid (IC 50 : 1,69 µg/mL), trans-2-hexenyl gallate (IC 50 : 2,79 µg/mL), and benzyl gallate (IC 50 : 2,98 µg/mL). In general, the results indicate that addition of alkyl ester chain on gallic acid can increase its cytotoxicity against HeLa cells.
Keywords : gallic acid, alkyl gallate ester, alkyl gallate ether, HeLa cells