Skripsi

Evaluation of Formalin, CaCl2, and Glycerin as Advanced Preservative Solutions to the Structures of Brain Tissues = Evaluasi Formalin, CaCl2, dan Gliserin sebagai Larutan Pengawet Lanjutan terhadap Struktur Jaringan Otak.

Formalin is the main preservative solution in preserving cadavers used for educational purposes. Even though formalin is proven to be effective in preserving cadavers, there are some harmful effects such as carcinogenic, toxigenic, and caused an irritation to the eyes and nose. That is why it is needed to look for other advanced preservative solution to replace or decrease the usage of formalin. This research is to evaluate the effect of formalin substitution candidates, which are CaCl2 and glycerin as advanced preservative solutions in preserving brain tissues. Steps were done were preserving the brain with the initial fixation, either formalin 10% or formalin 25%, then divided into four groups to be preserved with four types of advanced preservative solution, which is formalin controlled solution (standard preservative solution by Department of Anatomy, Faculty of Medicine Universitas Indonesia), 15% CaCl2, 20% CaCl2, and 70% glycerin + 0.1% thymol in ethanol. Observation and analysis were done on macroscopic structure (tissue consistency and presence of fungi) and microscopic structure (necrotic percentage and tissue abnormality). Macroscopic result showed that brains that were preserved in 15% CaCl2 had mushy condition with presence of surface fungi in the solutions. In microscopic examination, there were significant differences between four solutions with 70% glycerin + 0.1% thymol in ethanol showed the best result to preserve brain tissues (p