Skripsi

The Influence of Exposure Time to Continuous Systemic Hypoxia on Lung Reduced-Glutathione (GSH) Level in Rats = Pengaruh Waktu Pemaparan Hipoksia Sistemik Kontinu terhadap Kadar Glutation Tereduksi (GSH) Paru pada Tikus.

Background: Reduced-glutathione (GSH) is a major endogenous nonenzymatic antioxidant in the lung and airway system. GSH oxidizes reactive oxygen species (ROS) to prevent oxidative damage. Hence, GSH is considered one of the parameters for measuring the degree of ROS-induced oxidative stress. Continuous systemic hypoxia has been known to cause ROS formation and oxidative damage. Consequently, this research attempted to see the effect of exposure time to continuous systemic hypoxia to ROS formation in the lung as reflected by GSH level. Methods: Lung samples were collected from 6-8 weeks old male Sprague-Dawley rats weighing 150-200g, previously exposed to normoxic environment (control) or continuous systemic hypoxia (10% O2, 90%N) for 1, 3, 5, or 7 days. Afterwards, GSH level was measured from lung extracts. Results: Data analysis using ANOVA indicated a significant difference in lung GSH level upon different exposure times to continuous systemic hypoxia (p=0.002). Post hoc LSD comparisons revealed that hypoxic exposure should be of at least 5 days to yield an effect, as shown by significantly reduced GSH level in hypoxic groups of 5 days (2.197±0.284, p=0.001) and 7 days (2.294±0.445, p=0.001). Meanwhile, hypoxic exposure for 3 days or less did not significantly affect GSH level. Further Pearson’s correlation analysis demonstrated a very strong negative relationship between hypoxic exposure times and lung GSH level (r=-0.854, p