Skripsi

Gene structure and tissue distribution of RIKEN cDNA 17000G05 in the mouse epididymis. = Struktur gen dan distribusi jaringan dari gen RIKEN cDNA 17000G05 di epididimis mencit.

Nowadays, male role in contraceptive methods is considerably low. The development of a non-hormonal male contraception might reduce the number of human population. Epididymis secretes numerous proteins that affect sperm maturation process. These proteins are expressed by epididymal genes. If the activity of these genes is inactivated, the sperm maturation process might also be halted. Therefore, epididymal genes are potential candidates for male contraceptive target. One of the genes that located in the epididymis is RIKEN cDNA 1700018G05. Our research has a goal to characterize RIKEN cDNA 1700018G05, especially to analyze the gene structure and tissue distribution. The research was done at Biology Department, Faculty of Medicine Universitas Indonesia, starting from May 2012 until February 2013, using two major analysis, which are tissue distribution analysis and bioinformatics analysis. The result shows that RIKEN cDNA 1700018G05 is more distributed in non-epididymal tissue. Therefore the usage of this gene for non-hormonal male contraception need to be reconsidered.
Key words: Epididymis, sperm, RIKEN cDNA 17000G05, contraception.


Dewasa ini, peran kaum pria dalam keluarga berencana tergolong cukup rendah. Pengembangan kontrasepsi pria non-hormonal akan meningkatkan peran pria dalam keluarga berencana. Epididymis mensekresikan banyak protein yang berperan dalam proses maturasi sperma di epididymis. Protein – protein tersebut di expresikan oleh gen yang berada di epididymis. Bila aktivitas dari gen – gen ini dihentikan, proses maturasi sperma juga akan terganggu. Salah satu gen yang berada di epididymis adalah RIKEN cDNA 1700018G05. Penelitian ini bertujuan untuk mengkarakterisasi gen RIKEN cDNA 1700018G05, terutama untuk mengetahui distribusi jaringan dari gen tersebut. Penelitian ini dilaksanakan di Departemen Biologi Fakultas Kedokteran Universitas Indonesia, dimulai sejak bulan Mei 2012 hingga Februari 2013, dengan dua analisa, yaitu analisis distribusi jaringan dan analisis bioinformatika. Hasil penelitian menunjukkan bahwa pendistribuasian RIKEN cDNA 1700018G05 lebih banyak di jaringan nonepididimis.
Sehingga penggunaan RIKEN cDNA 1700018G05 untuk kontrasepsi pria non-hormonal perlu dipertimbangkan kembali.
Kata kunci: Epididimis, sperma, RIKEN cDNA 17000G05, kontrasepsi.