Tesis

Efek Pemberian Tetes Mata Hydroksypropyl-Guar terhadap Densitas Sel Goblet, Stabilitas Lapisan Air Mata, dan Interleukin 6 Pasca Peritomi Konjungtiva 360 Derajat = The Effect of Hydroxypropyl-guar on Goblet Cell Density, Tear Film Stability, and Interleukin 6 after Conjunctival Peritomy 360 Degree

Latar Belakang Tindakan peritomi konjungtiva meningkatkan penanda inflamasi pada permukaan okular dan menyebabkan penurunan densitas sel goblet yang berimplikasi pada ketidakstabilan lapisan air mata. Pemberian HP-guar diharapkan mampu memperbaiki keadaan permukaan okular pasca peritomi konjungtiva 360° dibandingkan kontrol. Tujuan Untuk menilai efektivitas artificial tears HP-guar dalam melindungi permukaan okular pasien pasca peritomi konjungtiva 360°. Desain Penelitian prospektif, uji klinis open label Hasil Terdapat 23 subjek yang berpartisipasi dalam penelitian ini. Kelompok HP-guar menunjukkan hasil yang lebih baik dibandingkan dengan kelompok kontrol secara signifikan dalam hal TFBUT (9,43+2,20 vs 5,95+1,93, p=0,001), densitas sel goblet (68,73 ±97,49 vs 10,00 ± 22,09,p =0,012), skor epitel (1,73±1.00 vs 4,55±1.57, p=0.000). Tidak terdapat perbedaan bermakna antara kedua kelompok dalam hal skor lissamine green (1,33±1,55 vs 2,18±1,72, p=0,146) dan IL-6 air mata (60,32 ±17,86 vs 57,44 ±24,67, p=0,782) Kesimpulan Pemberian HP-guar lebih memperbaiki nilai TFBUT, densitas sel goblet dan epitel konjungtiva dibandingkan kontrol. HP-guar tidak berpengaruh terhadap skor lissamine green dan kadar IL-6 air mata.
Kata kunci Peritomi konjungtiva, Hydroksypropyl-Guar, densitas sel goblet, TFBUT, IL-6, lissamine green, permukaan okular


Background Conjunctival peritomy, a step commonly done in ocular surgery, increases ocular surface inflammation and decreases goblet cell density (GCD) that could lead to tear film instability. Hydroxypropyl-guar (HP-guar) is expected to improve ocular surface related to conjunctival peritomy Purpose To evaluate HP-guar effectiveness in protecting ocular surface after conjunctival peritomy Methods Randomized controlled trial, open label study, on subjects underwent scleral buckling surgery Result Twenty-three participants were involved in this study. The HP-guar group showed statistically better results compared to the control group regarding Tear-film break-up time (TFBUT) (9,43±2,20 vs 5,95±1,93, p=0.001), GCD (68,73 ±97,49 vs 10,00 ± 22,09,p =0.012), and epithelial score (1.73±1.00 vs 4.55±1.57, p=0.000). No statistical differences were found between groups in lissamine green score (1.33±1.55 vs 2.18±1.72, p=0.146) and IL-6 tear fluid level (60.32 ±17.86 vs 57.44 ±24.67, p=0.782) Conclusion The addition of HP-Guar to regular treatment after conjunctival peritomy increases TFBUT, goblet cell density, and improves conjunctival epithelial cells. HPguar has no effect on ocular surface inflammation.
Keywords Conjunctival peritomy, hydroxypropyl-guar, goblet cell density, TFBUT, IL-6, lissamine green, ocular surface