Skripsi

Evaluationof bio-rad NS1 ag strip as diagnostic kit for denv-2, denv-4 and mixed acute dengue infection in indonesia. = Evaluasi Bio-Rad NS1 Ag Strip sebagai Alat Diagnostik Infeksi Dengue AkutDENV-2, DENV-4 dan Campuran di Indonesia.

Non Structural-1 (NS1) protein of dengue virus is proven to be a marker for early diagnosis of dengue infection. Bio-Rad NS1 Ag Strip is one of the available diagnostic kit in Indonesia that comprised of NS1. The aim of this study was to measure the sensitivity and specificity of the kit within DENV-2, DENV-4and mixed dengue virus serotypes. This study was done in 36 months (March 2010- February 2013). There were 102 dengue suspected patients with fever less than 48 hours was fulfilling inclusion criteria. Reverse Transcription- Polymerase Chain Reaction (RT-PCR) or virus isolation in C6/36 cell line or increase titer antibody by ELISA was used as gold standard. RT-PCR was also used to determine serotype of the dengue virus. SPSS version 17.0 was the main statistical tool that we used. Data binominal variables was presented as incidence rates with 95% confidence intervals. Sensitivity and specificity of diagnostic kit was presented in 2x2 tablesand area under the curve (AUC) of the Receiver Operating Characteristics Curve. From 102 patients, 68 (68.3%) patients were confirmed positive dengue infection. Within confirmed dengue infection patients by RT-PCR we found DENV-1, DENV-2, DENV-3,DENV-4 17 (16.7%), 21 (20.5%), 16 (15.7%), 4 (3.9%), respectively. It was also found some mixed infection cases, which were (i)DENV-1 and DENV-2, (ii)DENV-1 and DENV-3, (iii)DENV-1 and DENV-4, (iv)DENV-1, DENV-3 and DENV-4, (v)DENV-2 and DENV-4 and (vi)unknown were of 2 (2.0%), 3 (2.9%), 1 (1.0%), 1 (1.0%), 1 (1.0%) and 2 (2.0%), respectively. The sensitivity and specificity of Bio-Rad NS1 Ag Strip for DENV-2 sera collected were 76.2% and 100% (95% CI, 76.8% to 99.3%), respectively. The sensitivity and specificity of Bio-Rad NS1 Ag Strip for DENV-4 were 50% and 100% (95% CI, 50% to 100%), respectively. The sensitivity and specificity of Bio-Rad NS1 Ag Strip for mixed infection were both 100% (95% CI, 95% to 100%). Bio-Rad NS1 Ag Strip has high sensitivity and specificity to determine DENV-2 and mixed infection. On the other hand, Bio-Rad NS1 Ag Strip has limited sensitivity, but high specificity for diagnosis of DENV-4 acute infection.
Keywords: Bio-Rad, DENV-2, DENV-4, mixed, NS1, sensitivity, specificity.