Skripsi

The effect of temperature on the proliferation and mitotic count of the mice adenocarcinoma mammae cells

ABSTRACT : The incidence of breast cancer patients was considered high both in the world and in Indonesia. An in vitro study stated that the malignant human endometrial adenocarcinoma cells exposed to more than 40.0°C for 24 hours were drastically damaged. The aim of this research is to analyze the temperature effect on the proliferation of adenocarcinoma mammae cells in vivo. A true experimental design is used with C3H mice exposed to: 20-22°C, 25-27°C, 32-34°C, and 37-39°C for 6 hours in 2 weeks. However, the study of group 37-39°C was not continued because the mice failed to survive. Two kinds of staining were used, AgNORs for cells proliferation and Hematoxylin-Eosin for mitotic count. AgNORs result revealed that the mAgNORs and pAgNORs of group 32-34°C was the highest and the differences are significant with p = 0,000 through One-Way ANOVA. HE result showed that there was a tendency of group 32-34°C to have a higher mitotic count compared to the other two groups but was insignificant through Mann-Whitney. The result of the AgNORs study is expected to give an idea for breast cancer non-invasive therapy by manipulating environmental temperature.
Keywords: temperature, cells proliferation, mitotic count, adenocarcinoma mammae, AgNORs.