Skripsi

The effect of environmental temperature on the mice adenocarcinoma mammae cell proliferation and the relation with lymphocyte count

Based on an in vitro study, it has been reported that there is an effect of temperature on the proliferation and malignant human endometrial adenocarcinoma cells. Thus, the aim of this research is identify whether temperature has effect on proliferation in the tumor cell (adenocarcinoma) especially in vivo condition. The experiment was conducted in true experimental design (parallel), in which C3H mice are used, arranged in three cages with different temperatures: 20-22°C, 25-27°C, and 32- 34°C in 2 weeks every day for 6 hours each day. The proliferation of the cells was detected with Argyrophilic Nucleolar Organizer Region (AgNOR) staining (mAgNOR and pAgNOR) and lymphocytes were stained with Hematoxylin-Eosin staining. As the result, One-Way ANOVA analysis shows p = 0,000 for mAgNOR and p = 0,000 for pAgNOR. In addition, Mann-Whitney analysis for lymphocyte counts shows p = 0,0241 for both 20-22°C & 25-27°C and 25-27°C & 32-34°C. In conclusion, temperature affects cell proliferation in vivo. However, there is no difference in lymphocyte counts after exposure with those temperatures. This result might contribute to options for non- pharmacological cancer therapy by controlling the environment temperature.
Key words: adenocarcinoma mamae, cells proliferation, lymphocyte, temperature, AgNORs, Hematoxylin-Eosin.