Skripsi

Envelope Amino Acid Sequence Comparison between Dengue Virus type 2 Isolated in Cipto Mangunkusumo Hospital Jakarta in 2006 and Nine other Countries. = Perbandingan Sekuens Selubung Asam Amino Antara Virus Dengue Tipe 2 Isolat Rumah Sakit Umum Pusat Nasional Cipto Mangunkusumo Jakarta pada Tahun 2006 Dengan Sembilan Negara Lainnya.

This research is aimed to obtain the envelope nucleotide and peptide sequence of dengue virus type 2 strain DS 20/06 Jakarta and compare it to other envelope sequences from Australia, Brunei Darussalam, Cambodia, China, India, Puerto Rico, Singapore, Thailand, and Vietnam; and to predict the relationship between the strains, which include discovering the genotype for each one. To obtain the full envelope nucleotide and peptide sequence from the Jakarta strain, PCR method was employed and Sanger method was done for the sequencing. The sequences were compared using the program Genetyx 5.1. From the comparison between strains in the nucleotide homology, our strain was closest to the Australian strain. In amino acid homology, DS 20/06 was closest to the Singapore (99.6%) and Australian (99.6%) strains. From the cladogram, it was revealed that strain from Indonesia belongs to the Cosmopolitan genotype.
Keywords: dengue, envelope protein sequence.



Penilitian ini ditujukan untuk mendapatkan sekuens selubung asam amino dan peptida virus dengue tipe 2 strain DS 20/06 Jakarta dan membandingkannya dengan sekuens protein selubung lain dari Australia, Brunei Darussalam, Kamboja, Cina, India, Puerto Rico, Singapura, Thailand, dan Vietnam; dan untuk memprediksikan hubungan antar strain, termasuk didalamnya mengklasifikasikan setiap strain tersebut. Metode PCR di gunakan untuk mendapatkan sekuens lengkap protein selubung dari strain DS 20/06 Jakarta dan metode Sanger untuk sequencing. Hasil sequencing dibandingkan menggunakan program Genetyx 5.1. Dari perbandingan homologi nukleotida antar strain, strain DS 20/06 paling mendekati strain Australia. Pada perbandingan homologi asam amino, strain DS 20/06 paling mendekati strain Australia (99.6%) dan Singapura (99.6%). Dari hasil kladogram memperlihatkan bahwa strain yang berasal dari Jakarta merupakan genotip Cosmopolitan.
Kata kunci: dengue, sekuens protein selubung.